Parental High-Fat Diet Promotes Inflammatory and Senescence-Related Changes in Prostate

الملخص EN

Background.

Obesity and dietary habits are associated with increased incidences of aging-related prostatic diseases.

The present study was aimed to investigate transgenerational effects of chronic high-fat diet (HFD) feeding on inflammation and senescence-related changes in prostate.

Methods.

Sprague-Dawley rats were kept on either normal or HFD one.

Senescence-associated β-galactosidase (SA β-gal) activity, inflammation, and cellular proliferation were determined in the prostate.

Results.

Increased SA β-gal activity, expression of p53, and cell proliferation marker PCNA were observed in ventral prostate of HFD-fed rats.

Immunostaining for p53 and PCNA revealed that the p53 immunopositive cells were primarily in stroma while PCNA immunopositive cells were epithelial cells.

An increase in expression of cycloxygenase-2 (COX-2) and phosphorylation of nuclear factor-kappa B (NF-kB) was observed in prostate of weaning pups HFD-fed parents.

However, in adult pups, irrespective of dietary habit, a significant increase in the expression of COX-2, PCNA, phosphorylation of NF-kB, infiltration of inflammatory cells, and SA β-gal activity was observed.

Conclusions.

Present investigation reports that HFD feeding promotes accumulation of p53 expressing cells, proliferation of epithelial cells, and senescence-related changes in prostate.

Further, parental HFD-feeding upholds inflammatory, proliferative, and senescence-related changes in prostate of pups.