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Cultivation and Immortalization of Human B-Cells Producing a Human Monoclonal IgM Antibody Binding to MDA-LDL: Further Evidence for Formation of Atherogenic MDA-LDL Adducts in Humans In Vivo
المؤلفون المشاركون
Holasek, Sandra
Tatzber, Franz
Pursch, Edith
Resch, Ulrike
Pfragner, Roswitha
Lindschinger, Meinrad
Cvirn, Gerhard
Wonisch, Willibald
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-11-09
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Oxidatively modified low-density lipoprotein (oLDL) is firmly believed to play an important role in the initiation and development of atherosclerosis, and malonic dialdehyde (MDA) is one of the major lipid peroxidation breakdown products involved in this process.
In recent decades, antibodies against MDA-LDL have been detected in human and animal sera.
In our study, human B-cells from the peripheral blood of a healthy female donor were fused with the SP2/0 mouse myeloma cell line.
Antibody-producing hybridomas were detected by MDA-LDL-IgG/IgM enzyme-linked immunosorbent assays (ELISA) and Cu++-oxidized LDL IgG/IgM (oLAb) ELISA.
Cells with supernatants emitting positive signals for antibodies were then cloned and after sufficient multiplication frozen and stored under liquid nitrogen.
Due to the loss of antibody-producing ability, we established an MDA-LDL-IgM-producing cell line by recloning.
This allowed isolation and immortalization of several human B-cells.
The human donor had not been immunized with MDA-modified proteins, thus obviously producing MDA-LDL antibodies in vivo.
Furthermore, using these antibodies for in vitro experiments, we were able to demonstrate that MDA epitopes are among the epitopes generated during Cu++-LDL oxidation as well.
Finally, these antibodies compete in ELISA and cell culture experiments with MDA as a challenging toxin or ligand.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tatzber, Franz& Pursch, Edith& Resch, Ulrike& Pfragner, Roswitha& Holasek, Sandra& Lindschinger, Meinrad…[et al.]. 2017. Cultivation and Immortalization of Human B-Cells Producing a Human Monoclonal IgM Antibody Binding to MDA-LDL: Further Evidence for Formation of Atherogenic MDA-LDL Adducts in Humans In Vivo. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1195134
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tatzber, Franz…[et al.]. Cultivation and Immortalization of Human B-Cells Producing a Human Monoclonal IgM Antibody Binding to MDA-LDL: Further Evidence for Formation of Atherogenic MDA-LDL Adducts in Humans In Vivo. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1195134
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tatzber, Franz& Pursch, Edith& Resch, Ulrike& Pfragner, Roswitha& Holasek, Sandra& Lindschinger, Meinrad…[et al.]. Cultivation and Immortalization of Human B-Cells Producing a Human Monoclonal IgM Antibody Binding to MDA-LDL: Further Evidence for Formation of Atherogenic MDA-LDL Adducts in Humans In Vivo. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1195134
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1195134
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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