Cultivation and Immortalization of Human B-Cells Producing a Human Monoclonal IgM Antibody Binding to MDA-LDL: Further Evidence for Formation of Atherogenic MDA-LDL Adducts in Humans In Vivo
Joint Authors
Holasek, Sandra
Tatzber, Franz
Pursch, Edith
Resch, Ulrike
Pfragner, Roswitha
Lindschinger, Meinrad
Cvirn, Gerhard
Wonisch, Willibald
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-09
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Oxidatively modified low-density lipoprotein (oLDL) is firmly believed to play an important role in the initiation and development of atherosclerosis, and malonic dialdehyde (MDA) is one of the major lipid peroxidation breakdown products involved in this process.
In recent decades, antibodies against MDA-LDL have been detected in human and animal sera.
In our study, human B-cells from the peripheral blood of a healthy female donor were fused with the SP2/0 mouse myeloma cell line.
Antibody-producing hybridomas were detected by MDA-LDL-IgG/IgM enzyme-linked immunosorbent assays (ELISA) and Cu++-oxidized LDL IgG/IgM (oLAb) ELISA.
Cells with supernatants emitting positive signals for antibodies were then cloned and after sufficient multiplication frozen and stored under liquid nitrogen.
Due to the loss of antibody-producing ability, we established an MDA-LDL-IgM-producing cell line by recloning.
This allowed isolation and immortalization of several human B-cells.
The human donor had not been immunized with MDA-modified proteins, thus obviously producing MDA-LDL antibodies in vivo.
Furthermore, using these antibodies for in vitro experiments, we were able to demonstrate that MDA epitopes are among the epitopes generated during Cu++-LDL oxidation as well.
Finally, these antibodies compete in ELISA and cell culture experiments with MDA as a challenging toxin or ligand.
American Psychological Association (APA)
Tatzber, Franz& Pursch, Edith& Resch, Ulrike& Pfragner, Roswitha& Holasek, Sandra& Lindschinger, Meinrad…[et al.]. 2017. Cultivation and Immortalization of Human B-Cells Producing a Human Monoclonal IgM Antibody Binding to MDA-LDL: Further Evidence for Formation of Atherogenic MDA-LDL Adducts in Humans In Vivo. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1195134
Modern Language Association (MLA)
Tatzber, Franz…[et al.]. Cultivation and Immortalization of Human B-Cells Producing a Human Monoclonal IgM Antibody Binding to MDA-LDL: Further Evidence for Formation of Atherogenic MDA-LDL Adducts in Humans In Vivo. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1195134
American Medical Association (AMA)
Tatzber, Franz& Pursch, Edith& Resch, Ulrike& Pfragner, Roswitha& Holasek, Sandra& Lindschinger, Meinrad…[et al.]. Cultivation and Immortalization of Human B-Cells Producing a Human Monoclonal IgM Antibody Binding to MDA-LDL: Further Evidence for Formation of Atherogenic MDA-LDL Adducts in Humans In Vivo. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1195134
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1195134