Nicotinamide Adenine Dinucleotide Protects against Spinal Cord Ischemia Reperfusion Injury-Induced Apoptosis by Blocking Autophagy
المؤلفون المشاركون
Li, Changwei
Xie, Lei
Yu, Sifei
Wang, Zhenfei
Yang, Kai
Liu, Zhuochao
Liang, Yu
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-03-07
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The role of autophagy, neuroprotective mechanisms of nicotinamide adenine dinucleotide (NAD+), and their relationship in spinal cord ischemic reperfusion injury (SCIR) was assessed.
Forty-eight Sprague-Dawley rats were divided into four groups: sham, ischemia reperfusion (I/R), 10 mg/kg NAD+, and 75 mg/kg NAD+.
Western blotting, immunofluorescence, and immunohistochemistry were used to assess autophagy and apoptosis.
Basso, Beattie, and Bresnahan (BBB) scores were used to assess neurological function.
Expression levels of Beclin-1, Atg12-Atg5, LC3B-II, cleaved caspase 3, and Bax were upregulated in the I/R group and downregulated in the 75 mg/kg NAD+ group; p-mTOR, p-AKT, p62, and Bcl-2 were downregulated in the I/R group and upregulated in the 75 mg/kg NAD+ group.
Numbers of LC3B-positive, caspase 3-positive, Bax-positive, and TUNEL-positive cells were significantly increased in the I/R group and decreased in the 75 mg/kg NAD+ group.
The mean integrated option density of Bax increased and that of Nissl decreased in the I/R group, and it decreased and increased, respectively, in the 75 mg/kg NAD+ group.
BBB scores significantly increased in the 75 mg/kg NAD+ group relative to the I/R group.
No difference was observed between I/R and 10 mg/kg NAD+ groups for these indicators.
Therefore, excessive and sustained autophagy aggravates SCIR; administration of NAD+ alleviates injury.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xie, Lei& Yu, Sifei& Wang, Zhenfei& Yang, Kai& Liu, Zhuochao& Li, Changwei…[et al.]. 2017. Nicotinamide Adenine Dinucleotide Protects against Spinal Cord Ischemia Reperfusion Injury-Induced Apoptosis by Blocking Autophagy. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1195427
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xie, Lei…[et al.]. Nicotinamide Adenine Dinucleotide Protects against Spinal Cord Ischemia Reperfusion Injury-Induced Apoptosis by Blocking Autophagy. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1195427
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xie, Lei& Yu, Sifei& Wang, Zhenfei& Yang, Kai& Liu, Zhuochao& Li, Changwei…[et al.]. Nicotinamide Adenine Dinucleotide Protects against Spinal Cord Ischemia Reperfusion Injury-Induced Apoptosis by Blocking Autophagy. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1195427
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1195427
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر