Nicotinamide Adenine Dinucleotide Protects against Spinal Cord Ischemia Reperfusion Injury-Induced Apoptosis by Blocking Autophagy
Joint Authors
Li, Changwei
Xie, Lei
Yu, Sifei
Wang, Zhenfei
Yang, Kai
Liu, Zhuochao
Liang, Yu
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The role of autophagy, neuroprotective mechanisms of nicotinamide adenine dinucleotide (NAD+), and their relationship in spinal cord ischemic reperfusion injury (SCIR) was assessed.
Forty-eight Sprague-Dawley rats were divided into four groups: sham, ischemia reperfusion (I/R), 10 mg/kg NAD+, and 75 mg/kg NAD+.
Western blotting, immunofluorescence, and immunohistochemistry were used to assess autophagy and apoptosis.
Basso, Beattie, and Bresnahan (BBB) scores were used to assess neurological function.
Expression levels of Beclin-1, Atg12-Atg5, LC3B-II, cleaved caspase 3, and Bax were upregulated in the I/R group and downregulated in the 75 mg/kg NAD+ group; p-mTOR, p-AKT, p62, and Bcl-2 were downregulated in the I/R group and upregulated in the 75 mg/kg NAD+ group.
Numbers of LC3B-positive, caspase 3-positive, Bax-positive, and TUNEL-positive cells were significantly increased in the I/R group and decreased in the 75 mg/kg NAD+ group.
The mean integrated option density of Bax increased and that of Nissl decreased in the I/R group, and it decreased and increased, respectively, in the 75 mg/kg NAD+ group.
BBB scores significantly increased in the 75 mg/kg NAD+ group relative to the I/R group.
No difference was observed between I/R and 10 mg/kg NAD+ groups for these indicators.
Therefore, excessive and sustained autophagy aggravates SCIR; administration of NAD+ alleviates injury.
American Psychological Association (APA)
Xie, Lei& Yu, Sifei& Wang, Zhenfei& Yang, Kai& Liu, Zhuochao& Li, Changwei…[et al.]. 2017. Nicotinamide Adenine Dinucleotide Protects against Spinal Cord Ischemia Reperfusion Injury-Induced Apoptosis by Blocking Autophagy. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1195427
Modern Language Association (MLA)
Xie, Lei…[et al.]. Nicotinamide Adenine Dinucleotide Protects against Spinal Cord Ischemia Reperfusion Injury-Induced Apoptosis by Blocking Autophagy. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1195427
American Medical Association (AMA)
Xie, Lei& Yu, Sifei& Wang, Zhenfei& Yang, Kai& Liu, Zhuochao& Li, Changwei…[et al.]. Nicotinamide Adenine Dinucleotide Protects against Spinal Cord Ischemia Reperfusion Injury-Induced Apoptosis by Blocking Autophagy. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1195427
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1195427