MitoTEMPO Prevents Oxalate Induced Injury in NRK-52E Cells via Inhibiting Mitochondrial Dysfunction and Modulating Oxidative Stress
المؤلفون المشاركون
Liu, Jihong
Wang, Shaogang
Zhang, Jiaqiao
Wang, Qing
Xu, Chuou
Lu, Yuchao
Hu, Henglong
Qin, Baolong
Wang, Yufeng
He, Deng
Li, Cong
Yu, Xiao
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-01-02
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
As one of the major risks for urolithiasis, hyperoxaluria can be caused by genetic defect or dietary intake.
And high oxalate induced renal epithelial cells injury is related to oxidative stress and mitochondrial dysfunction.
Here, we investigated whether MitoTEMPO, a mitochondria-targeted antioxidant, could protect against oxalate mediated injury in NRK-52E cells via inhibiting mitochondrial dysfunction and modulating oxidative stress.
MitoSOX Red was used to determine mitochondrial ROS (mtROS) production.
Mitochondrial membrane potential (Δψm) and quantification of ATP synthesis were measured to evaluate mitochondrial function.
The protein expression of Nox4, Nox2, and p22 was also detected to explore the effect of oxalate and MitoTEMPO on NADPH oxidase.
Our results revealed that pretreatment with MitoTEMPO significantly inhibited oxalate induced lactate dehydrogenase (LDH) and malondialdehyde (MDA) release and decreased oxalate induced mtROS generation.
Further, MitoTEMPO pretreatment restored disruption of Δψm and decreased ATP synthesis mediated by oxalate.
In addition, MitoTEMPO altered the protein expression of Nox4 and p22 and decreased the protein expression of IL-6 and osteopontin (OPN) induced by oxalate.
We concluded that MitoTEMPO may be a new candidate to protect against oxalate induced kidney injury as well as urolithiasis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Jiaqiao& Wang, Qing& Xu, Chuou& Lu, Yuchao& Hu, Henglong& Qin, Baolong…[et al.]. 2017. MitoTEMPO Prevents Oxalate Induced Injury in NRK-52E Cells via Inhibiting Mitochondrial Dysfunction and Modulating Oxidative Stress. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1195690
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Jiaqiao…[et al.]. MitoTEMPO Prevents Oxalate Induced Injury in NRK-52E Cells via Inhibiting Mitochondrial Dysfunction and Modulating Oxidative Stress. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1195690
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Jiaqiao& Wang, Qing& Xu, Chuou& Lu, Yuchao& Hu, Henglong& Qin, Baolong…[et al.]. MitoTEMPO Prevents Oxalate Induced Injury in NRK-52E Cells via Inhibiting Mitochondrial Dysfunction and Modulating Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1195690
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1195690
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر