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MitoTEMPO Prevents Oxalate Induced Injury in NRK-52E Cells via Inhibiting Mitochondrial Dysfunction and Modulating Oxidative Stress
Joint Authors
Liu, Jihong
Wang, Shaogang
Zhang, Jiaqiao
Wang, Qing
Xu, Chuou
Lu, Yuchao
Hu, Henglong
Qin, Baolong
Wang, Yufeng
He, Deng
Li, Cong
Yu, Xiao
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
As one of the major risks for urolithiasis, hyperoxaluria can be caused by genetic defect or dietary intake.
And high oxalate induced renal epithelial cells injury is related to oxidative stress and mitochondrial dysfunction.
Here, we investigated whether MitoTEMPO, a mitochondria-targeted antioxidant, could protect against oxalate mediated injury in NRK-52E cells via inhibiting mitochondrial dysfunction and modulating oxidative stress.
MitoSOX Red was used to determine mitochondrial ROS (mtROS) production.
Mitochondrial membrane potential (Δψm) and quantification of ATP synthesis were measured to evaluate mitochondrial function.
The protein expression of Nox4, Nox2, and p22 was also detected to explore the effect of oxalate and MitoTEMPO on NADPH oxidase.
Our results revealed that pretreatment with MitoTEMPO significantly inhibited oxalate induced lactate dehydrogenase (LDH) and malondialdehyde (MDA) release and decreased oxalate induced mtROS generation.
Further, MitoTEMPO pretreatment restored disruption of Δψm and decreased ATP synthesis mediated by oxalate.
In addition, MitoTEMPO altered the protein expression of Nox4 and p22 and decreased the protein expression of IL-6 and osteopontin (OPN) induced by oxalate.
We concluded that MitoTEMPO may be a new candidate to protect against oxalate induced kidney injury as well as urolithiasis.
American Psychological Association (APA)
Zhang, Jiaqiao& Wang, Qing& Xu, Chuou& Lu, Yuchao& Hu, Henglong& Qin, Baolong…[et al.]. 2017. MitoTEMPO Prevents Oxalate Induced Injury in NRK-52E Cells via Inhibiting Mitochondrial Dysfunction and Modulating Oxidative Stress. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1195690
Modern Language Association (MLA)
Zhang, Jiaqiao…[et al.]. MitoTEMPO Prevents Oxalate Induced Injury in NRK-52E Cells via Inhibiting Mitochondrial Dysfunction and Modulating Oxidative Stress. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1195690
American Medical Association (AMA)
Zhang, Jiaqiao& Wang, Qing& Xu, Chuou& Lu, Yuchao& Hu, Henglong& Qin, Baolong…[et al.]. MitoTEMPO Prevents Oxalate Induced Injury in NRK-52E Cells via Inhibiting Mitochondrial Dysfunction and Modulating Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1195690
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1195690