Resveratrol Prevents ROS-Induced Apoptosis in High Glucose-Treated Retinal Capillary Endothelial Cells via the Activation of AMPKSirt1PGC-1α Pathway

المؤلفون المشاركون

Li, Jun
Yu, Songping
Ying, Jia
Shi, Tianyan
Wang, Peipei

المصدر

Oxidative Medicine and Cellular Longevity

العدد

المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2017-10-29

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الأحياء

الملخص EN

Resveratrol (RSV) is used as a protective therapy against diabetic retinopathy.

However, the mechanism(s) underlying this protective effect has not been fully elucidated.

Bovine retinal capillary endothelial cells (BRECs), an in vitro model, were used to investigate the mechanism of RSV.

Our results showed that high glucose induced significant cellular apoptosis in BRECs, which was accompanied by increased intracellular levels of reactive oxygen species (ROS) and cleaved caspase-3.

The glucose-induced apoptosis and ROS elevation were both inhibited by RSV.

High glucose was found to decrease the levels of phosphorylated AMP-activated protein kinase (p-AMPK), which was accompanied by increased levels of Sirt1 and PGC-1α.

These changes were reversed by RSV.

We also demonstrated that AMPK regulates the modulations of Sirt1 and PGC-1α using specific inhibitors of AMPK and Sirt1 and small interfering RNAs of PGC-1α.

In summary, the current study demonstrates that RSV is effective against high glucose-induced cellular apoptosis and its action is exerted via the inhibition of ROS/AMPK/Sirt1/PGC-1α pathway.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Li, Jun& Yu, Songping& Ying, Jia& Shi, Tianyan& Wang, Peipei. 2017. Resveratrol Prevents ROS-Induced Apoptosis in High Glucose-Treated Retinal Capillary Endothelial Cells via the Activation of AMPKSirt1PGC-1α Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1195700

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Li, Jun…[et al.]. Resveratrol Prevents ROS-Induced Apoptosis in High Glucose-Treated Retinal Capillary Endothelial Cells via the Activation of AMPKSirt1PGC-1α Pathway. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1195700

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Li, Jun& Yu, Songping& Ying, Jia& Shi, Tianyan& Wang, Peipei. Resveratrol Prevents ROS-Induced Apoptosis in High Glucose-Treated Retinal Capillary Endothelial Cells via the Activation of AMPKSirt1PGC-1α Pathway. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1195700

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1195700