Resveratrol Prevents ROS-Induced Apoptosis in High Glucose-Treated Retinal Capillary Endothelial Cells via the Activation of AMPKSirt1PGC-1α Pathway
Joint Authors
Li, Jun
Yu, Songping
Ying, Jia
Shi, Tianyan
Wang, Peipei
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Resveratrol (RSV) is used as a protective therapy against diabetic retinopathy.
However, the mechanism(s) underlying this protective effect has not been fully elucidated.
Bovine retinal capillary endothelial cells (BRECs), an in vitro model, were used to investigate the mechanism of RSV.
Our results showed that high glucose induced significant cellular apoptosis in BRECs, which was accompanied by increased intracellular levels of reactive oxygen species (ROS) and cleaved caspase-3.
The glucose-induced apoptosis and ROS elevation were both inhibited by RSV.
High glucose was found to decrease the levels of phosphorylated AMP-activated protein kinase (p-AMPK), which was accompanied by increased levels of Sirt1 and PGC-1α.
These changes were reversed by RSV.
We also demonstrated that AMPK regulates the modulations of Sirt1 and PGC-1α using specific inhibitors of AMPK and Sirt1 and small interfering RNAs of PGC-1α.
In summary, the current study demonstrates that RSV is effective against high glucose-induced cellular apoptosis and its action is exerted via the inhibition of ROS/AMPK/Sirt1/PGC-1α pathway.
American Psychological Association (APA)
Li, Jun& Yu, Songping& Ying, Jia& Shi, Tianyan& Wang, Peipei. 2017. Resveratrol Prevents ROS-Induced Apoptosis in High Glucose-Treated Retinal Capillary Endothelial Cells via the Activation of AMPKSirt1PGC-1α Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1195700
Modern Language Association (MLA)
Li, Jun…[et al.]. Resveratrol Prevents ROS-Induced Apoptosis in High Glucose-Treated Retinal Capillary Endothelial Cells via the Activation of AMPKSirt1PGC-1α Pathway. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1195700
American Medical Association (AMA)
Li, Jun& Yu, Songping& Ying, Jia& Shi, Tianyan& Wang, Peipei. Resveratrol Prevents ROS-Induced Apoptosis in High Glucose-Treated Retinal Capillary Endothelial Cells via the Activation of AMPKSirt1PGC-1α Pathway. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1195700
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1195700