Detection of Yersinia enterocolitica in Retail Chicken Meat, Mashhad, Iran
المؤلفون المشاركون
Jamshidi, Abdollah
Zeinali, Tayebeh
Sirghani, Khadigeh
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-4، 4ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-04-19
دولة النشر
مصر
عدد الصفحات
4
التخصصات الرئيسية
الملخص EN
Poultry meat is one of the most important sources of infection of Yersinia spp.
for humans.
The aim of the present study was to evaluate the incidence of Yersinia enterocolitica in chicken meat by using culture method on selective medium and confirmation by PCR assay.
Also, biochemical methods were used for biotyping.
A total of 100 chicken thigh meat samples were collected randomly from retail outlets in Mashhad, Iran.
Samples were enriched in Peptone-Sorbitol-Bile (PSB) broth and then cultured on Cefsulodin-Irgasan-Novobiocin (CIN) agar containing antibiotics supplement.
The DNA was extracted from suspected colonies of Yersinia spp.
and then PCR test using specific primers for 16S rRNA gene of Yersinia enterocolitica was performed.
In this study, 30% of chicken meat was contaminated with Yersinia spp.
by culture method and 25% of chicken meat was contaminated with Yersinia enterocolitica.
Biotyping of isolated colonies showed that all of the isolates belonged to biotype 1A.
Culture and detection of Yersinia spp.
from food samples traditionally take 4 days.
Due to high accuracy and speed of PCR assay, it is a good alternative method for microbiological techniques.
In conclusion, poultry meat can act as a source of Y.
enterocolitica and could be considered as a public health hazard.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sirghani, Khadigeh& Zeinali, Tayebeh& Jamshidi, Abdollah. 2018. Detection of Yersinia enterocolitica in Retail Chicken Meat, Mashhad, Iran. Journal of Pathogens،Vol. 2018, no. 2018, pp.1-4.
https://search.emarefa.net/detail/BIM-1197457
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sirghani, Khadigeh…[et al.]. Detection of Yersinia enterocolitica in Retail Chicken Meat, Mashhad, Iran. Journal of Pathogens No. 2018 (2018), pp.1-4.
https://search.emarefa.net/detail/BIM-1197457
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sirghani, Khadigeh& Zeinali, Tayebeh& Jamshidi, Abdollah. Detection of Yersinia enterocolitica in Retail Chicken Meat, Mashhad, Iran. Journal of Pathogens. 2018. Vol. 2018, no. 2018, pp.1-4.
https://search.emarefa.net/detail/BIM-1197457
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1197457
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر