Detection of Yersinia enterocolitica in Retail Chicken Meat, Mashhad, Iran
Joint Authors
Jamshidi, Abdollah
Zeinali, Tayebeh
Sirghani, Khadigeh
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-19
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Poultry meat is one of the most important sources of infection of Yersinia spp.
for humans.
The aim of the present study was to evaluate the incidence of Yersinia enterocolitica in chicken meat by using culture method on selective medium and confirmation by PCR assay.
Also, biochemical methods were used for biotyping.
A total of 100 chicken thigh meat samples were collected randomly from retail outlets in Mashhad, Iran.
Samples were enriched in Peptone-Sorbitol-Bile (PSB) broth and then cultured on Cefsulodin-Irgasan-Novobiocin (CIN) agar containing antibiotics supplement.
The DNA was extracted from suspected colonies of Yersinia spp.
and then PCR test using specific primers for 16S rRNA gene of Yersinia enterocolitica was performed.
In this study, 30% of chicken meat was contaminated with Yersinia spp.
by culture method and 25% of chicken meat was contaminated with Yersinia enterocolitica.
Biotyping of isolated colonies showed that all of the isolates belonged to biotype 1A.
Culture and detection of Yersinia spp.
from food samples traditionally take 4 days.
Due to high accuracy and speed of PCR assay, it is a good alternative method for microbiological techniques.
In conclusion, poultry meat can act as a source of Y.
enterocolitica and could be considered as a public health hazard.
American Psychological Association (APA)
Sirghani, Khadigeh& Zeinali, Tayebeh& Jamshidi, Abdollah. 2018. Detection of Yersinia enterocolitica in Retail Chicken Meat, Mashhad, Iran. Journal of Pathogens،Vol. 2018, no. 2018, pp.1-4.
https://search.emarefa.net/detail/BIM-1197457
Modern Language Association (MLA)
Sirghani, Khadigeh…[et al.]. Detection of Yersinia enterocolitica in Retail Chicken Meat, Mashhad, Iran. Journal of Pathogens No. 2018 (2018), pp.1-4.
https://search.emarefa.net/detail/BIM-1197457
American Medical Association (AMA)
Sirghani, Khadigeh& Zeinali, Tayebeh& Jamshidi, Abdollah. Detection of Yersinia enterocolitica in Retail Chicken Meat, Mashhad, Iran. Journal of Pathogens. 2018. Vol. 2018, no. 2018, pp.1-4.
https://search.emarefa.net/detail/BIM-1197457
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1197457