SEM Analysis of Surface Impact on Biofilm Antibiotic Treatment
المؤلفون المشاركون
Gomes, Luciana C.
Mergulhão, Filipe J.
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-01-11
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
The aim of this work was to use scanning electron microscopy (SEM) to investigate the effect of ampicillin treatment on Escherichia coli biofilms formed on two surface materials with different properties, silicone (SIL) and glass (GLA).
Epifluorescence microscopy (EM) was initially used to assess biofilm formation and killing efficiency on both surfaces.
This technique showed that higher bacterial colonization was obtained in the hydrophobic SIL than in the hydrophilic GLA.
It has also shown that higher biofilm inactivation was attained for GLA after the antibiotic treatment (7-log reduction versus 1-log reduction for SIL).
Due to its high resolution and magnification, SEM enabled a more detailed analysis of the antibiotic effect on biofilm cells, complementing the killing efficiency information obtained by EM.
SEM micrographs revealed that ampicillin-treated cells have an elongated form when compared to untreated cells.
Additionally, it has shown that different materials induced different levels of elongation on cells exposed to antibiotic.
Biofilms formed on GLA showed a 37% higher elongation than those formed on SIL.
Importantly, cell elongation was related to viability since ampicillin had a higher bactericidal effect on GLA-formed biofilms.
These findings raise the possibility of using SEM for understanding the efficacy of antimicrobial treatments by observation of biofilm morphology.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gomes, Luciana C.& Mergulhão, Filipe J.. 2017. SEM Analysis of Surface Impact on Biofilm Antibiotic Treatment. Scanning،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1197863
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gomes, Luciana C.& Mergulhão, Filipe J.. SEM Analysis of Surface Impact on Biofilm Antibiotic Treatment. Scanning No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1197863
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gomes, Luciana C.& Mergulhão, Filipe J.. SEM Analysis of Surface Impact on Biofilm Antibiotic Treatment. Scanning. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1197863
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1197863
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر