SEM Analysis of Surface Impact on Biofilm Antibiotic Treatment
Joint Authors
Gomes, Luciana C.
Mergulhão, Filipe J.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Information Technology and Computer Science
Abstract EN
The aim of this work was to use scanning electron microscopy (SEM) to investigate the effect of ampicillin treatment on Escherichia coli biofilms formed on two surface materials with different properties, silicone (SIL) and glass (GLA).
Epifluorescence microscopy (EM) was initially used to assess biofilm formation and killing efficiency on both surfaces.
This technique showed that higher bacterial colonization was obtained in the hydrophobic SIL than in the hydrophilic GLA.
It has also shown that higher biofilm inactivation was attained for GLA after the antibiotic treatment (7-log reduction versus 1-log reduction for SIL).
Due to its high resolution and magnification, SEM enabled a more detailed analysis of the antibiotic effect on biofilm cells, complementing the killing efficiency information obtained by EM.
SEM micrographs revealed that ampicillin-treated cells have an elongated form when compared to untreated cells.
Additionally, it has shown that different materials induced different levels of elongation on cells exposed to antibiotic.
Biofilms formed on GLA showed a 37% higher elongation than those formed on SIL.
Importantly, cell elongation was related to viability since ampicillin had a higher bactericidal effect on GLA-formed biofilms.
These findings raise the possibility of using SEM for understanding the efficacy of antimicrobial treatments by observation of biofilm morphology.
American Psychological Association (APA)
Gomes, Luciana C.& Mergulhão, Filipe J.. 2017. SEM Analysis of Surface Impact on Biofilm Antibiotic Treatment. Scanning،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1197863
Modern Language Association (MLA)
Gomes, Luciana C.& Mergulhão, Filipe J.. SEM Analysis of Surface Impact on Biofilm Antibiotic Treatment. Scanning No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1197863
American Medical Association (AMA)
Gomes, Luciana C.& Mergulhão, Filipe J.. SEM Analysis of Surface Impact on Biofilm Antibiotic Treatment. Scanning. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1197863
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1197863