Hepatoma-Derived Growth Factor Secreted from Mesenchymal Stem Cells Reduces Myocardial Ischemia-Reperfusion Injury
المؤلفون المشاركون
Wang, Yingchao
Zhang, Ling
Zhou, Yu
Chen, Panpan
Liu, Qingnian
Wu, Rongrong
Chen, Jinghai
Yu, Hong
Zhu, Wei
Hu, Xinyang
Wang, Jian-An
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-11-14
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
Objectives.
The present study aimed to explore the major factors that account for the beneficial effects of mesenchymal stem cells (MSCs).
Methods.
Using isobaric tags for relative and absolute quantitation method, hepatoma-derived growth factor (HDGF) was identified as an important factor secreted by MSCs, but not by cardiac fibroblasts (CFs).
The protective effects of conditioned medium (CdM) from MSCs or CFs were tested by using either H9C2 cells that were exposed by hypoxia-reoxygenation (H/R) insult or an in vivo mouse model of myocardial ischemia-reperfusion.
Results.
Compared to CF-CdM, MSC-CdM conferred protection against reperfusion injury.
CdM obtained from MSCs that were treated with HDGF-targeted shRNA failed to offer any protection in vitro.
In addition, administration of recombinant HDGF alone recapitulated the beneficial effects of MSC-CdM, which was associated with increased protein kinase C epsilon (PKCε) phosphorylation, enhanced mitochondria aldehyde dehydrogenase family 2 activity, and decreased 4-hydroxy-2-nonenal accumulation.
A significant decrease in infarct size and ameliorated cardiac dysfunction was achieved by administration of HDGF in wild-type mice, which was absent in PKCε dominant negative mice, indicating the essential roles of PKCε in HDGF-mediated protection.
Conclusions.
HDGF secreted from MSCs plays a key role in the protection against reperfusion injury through PKCε activation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhou, Yu& Chen, Panpan& Liu, Qingnian& Wang, Yingchao& Zhang, Ling& Wu, Rongrong…[et al.]. 2017. Hepatoma-Derived Growth Factor Secreted from Mesenchymal Stem Cells Reduces Myocardial Ischemia-Reperfusion Injury. Stem Cells International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1198077
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhou, Yu…[et al.]. Hepatoma-Derived Growth Factor Secreted from Mesenchymal Stem Cells Reduces Myocardial Ischemia-Reperfusion Injury. Stem Cells International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1198077
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhou, Yu& Chen, Panpan& Liu, Qingnian& Wang, Yingchao& Zhang, Ling& Wu, Rongrong…[et al.]. Hepatoma-Derived Growth Factor Secreted from Mesenchymal Stem Cells Reduces Myocardial Ischemia-Reperfusion Injury. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1198077
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1198077
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر