Hepatoma-Derived Growth Factor Secreted from Mesenchymal Stem Cells Reduces Myocardial Ischemia-Reperfusion Injury
Joint Authors
Wang, Yingchao
Zhang, Ling
Zhou, Yu
Chen, Panpan
Liu, Qingnian
Wu, Rongrong
Chen, Jinghai
Yu, Hong
Zhu, Wei
Hu, Xinyang
Wang, Jian-An
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-14
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Objectives.
The present study aimed to explore the major factors that account for the beneficial effects of mesenchymal stem cells (MSCs).
Methods.
Using isobaric tags for relative and absolute quantitation method, hepatoma-derived growth factor (HDGF) was identified as an important factor secreted by MSCs, but not by cardiac fibroblasts (CFs).
The protective effects of conditioned medium (CdM) from MSCs or CFs were tested by using either H9C2 cells that were exposed by hypoxia-reoxygenation (H/R) insult or an in vivo mouse model of myocardial ischemia-reperfusion.
Results.
Compared to CF-CdM, MSC-CdM conferred protection against reperfusion injury.
CdM obtained from MSCs that were treated with HDGF-targeted shRNA failed to offer any protection in vitro.
In addition, administration of recombinant HDGF alone recapitulated the beneficial effects of MSC-CdM, which was associated with increased protein kinase C epsilon (PKCε) phosphorylation, enhanced mitochondria aldehyde dehydrogenase family 2 activity, and decreased 4-hydroxy-2-nonenal accumulation.
A significant decrease in infarct size and ameliorated cardiac dysfunction was achieved by administration of HDGF in wild-type mice, which was absent in PKCε dominant negative mice, indicating the essential roles of PKCε in HDGF-mediated protection.
Conclusions.
HDGF secreted from MSCs plays a key role in the protection against reperfusion injury through PKCε activation.
American Psychological Association (APA)
Zhou, Yu& Chen, Panpan& Liu, Qingnian& Wang, Yingchao& Zhang, Ling& Wu, Rongrong…[et al.]. 2017. Hepatoma-Derived Growth Factor Secreted from Mesenchymal Stem Cells Reduces Myocardial Ischemia-Reperfusion Injury. Stem Cells International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1198077
Modern Language Association (MLA)
Zhou, Yu…[et al.]. Hepatoma-Derived Growth Factor Secreted from Mesenchymal Stem Cells Reduces Myocardial Ischemia-Reperfusion Injury. Stem Cells International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1198077
American Medical Association (AMA)
Zhou, Yu& Chen, Panpan& Liu, Qingnian& Wang, Yingchao& Zhang, Ling& Wu, Rongrong…[et al.]. Hepatoma-Derived Growth Factor Secreted from Mesenchymal Stem Cells Reduces Myocardial Ischemia-Reperfusion Injury. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1198077
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1198077