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Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro
المؤلفون المشاركون
Varghese, Divya S.
Parween, Shama
Ardah, Mustafa T.
Emerald, Bright Starling
Ansari, Suraiya A.
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-18، 18ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-09-24
دولة النشر
مصر
عدد الصفحات
18
الملخص EN
Human embryonic stem cells (hESCs) are being used extensively in array of studies to understand different mechanisms such as early human embryogenesis, drug toxicity testing, disease modeling, and cell replacement therapy.
The protocols for the directed differentiation of hESCs towards specific cell types often require long-term cell cultures.
To avoid bacterial contamination, these protocols include addition of antibiotics such as pen-strep and gentamicin.
Although aminoglycosides, streptomycin, and gentamicin have been shown to cause cytotoxicity in various animal models, the effect of these antibiotics on hESCs is not clear.
In this study, we found that antibiotics, pen-strep, and gentamicin did not affect hESC cell viability or expression of pluripotency markers.
However, during directed differentiation towards neural and hepatic fate, significant cell death was noted through the activation of caspase cascade.
Also, the expression of neural progenitor markers Pax6, Emx2, Otx2, and Pou3f2 was significantly reduced suggesting that gentamicin may adversely affect early embryonic neurogenesis whereas no effect was seen on the expression of endoderm or hepatic markers during differentiation.
Our results suggest that the use of antibiotics in cell culture media for the maintenance and differentiation of hESCs needs thorough investigation before use to avoid erroneous results.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Varghese, Divya S.& Parween, Shama& Ardah, Mustafa T.& Emerald, Bright Starling& Ansari, Suraiya A.. 2017. Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro. Stem Cells International،Vol. 2017, no. 2017, pp.1-18.
https://search.emarefa.net/detail/BIM-1200983
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Varghese, Divya S.…[et al.]. Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro. Stem Cells International No. 2017 (2017), pp.1-18.
https://search.emarefa.net/detail/BIM-1200983
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Varghese, Divya S.& Parween, Shama& Ardah, Mustafa T.& Emerald, Bright Starling& Ansari, Suraiya A.. Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-18.
https://search.emarefa.net/detail/BIM-1200983
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1200983
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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