Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro
Joint Authors
Varghese, Divya S.
Parween, Shama
Ardah, Mustafa T.
Emerald, Bright Starling
Ansari, Suraiya A.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-24
Country of Publication
Egypt
No. of Pages
18
Abstract EN
Human embryonic stem cells (hESCs) are being used extensively in array of studies to understand different mechanisms such as early human embryogenesis, drug toxicity testing, disease modeling, and cell replacement therapy.
The protocols for the directed differentiation of hESCs towards specific cell types often require long-term cell cultures.
To avoid bacterial contamination, these protocols include addition of antibiotics such as pen-strep and gentamicin.
Although aminoglycosides, streptomycin, and gentamicin have been shown to cause cytotoxicity in various animal models, the effect of these antibiotics on hESCs is not clear.
In this study, we found that antibiotics, pen-strep, and gentamicin did not affect hESC cell viability or expression of pluripotency markers.
However, during directed differentiation towards neural and hepatic fate, significant cell death was noted through the activation of caspase cascade.
Also, the expression of neural progenitor markers Pax6, Emx2, Otx2, and Pou3f2 was significantly reduced suggesting that gentamicin may adversely affect early embryonic neurogenesis whereas no effect was seen on the expression of endoderm or hepatic markers during differentiation.
Our results suggest that the use of antibiotics in cell culture media for the maintenance and differentiation of hESCs needs thorough investigation before use to avoid erroneous results.
American Psychological Association (APA)
Varghese, Divya S.& Parween, Shama& Ardah, Mustafa T.& Emerald, Bright Starling& Ansari, Suraiya A.. 2017. Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro. Stem Cells International،Vol. 2017, no. 2017, pp.1-18.
https://search.emarefa.net/detail/BIM-1200983
Modern Language Association (MLA)
Varghese, Divya S.…[et al.]. Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro. Stem Cells International No. 2017 (2017), pp.1-18.
https://search.emarefa.net/detail/BIM-1200983
American Medical Association (AMA)
Varghese, Divya S.& Parween, Shama& Ardah, Mustafa T.& Emerald, Bright Starling& Ansari, Suraiya A.. Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-18.
https://search.emarefa.net/detail/BIM-1200983
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1200983