Signal Factors Secreted by 2D and Spheroid Mesenchymal Stem Cells and by Cocultures of Mesenchymal Stem Cells Derived Microvesicles and Retinal Photoreceptor Neurons
المؤلفون المشاركون
Jiang, Bing
Xie, Lili
Mao, Mao
Zhou, Liang
Zhang, Lusi
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-01-18
دولة النشر
مصر
عدد الصفحات
13
الملخص EN
We aim to identify levels of signal factors secreted by MSCs cultured in 2D monolayers (2D-MSCs), spheroids (spheroids MSCs), and cocultures of microvesicles (MVs) derived from 2D-MSCs or spheroid MSCs and retinal photoreceptor neurons.
We seeded 2D-MSCs, spheroid MSCs, and cells derived from spheroids MSCs at equal numbers.
MVs isolated from all 3 culture conditions were incubated with 661W cells.
Levels of 51 signal factors in conditioned medium from those cultured conditions were quantified with bead-based assay.
We found that IL-8, IL-6, and GROα were the top three most abundant signal factors.
Moreover, compared to 2D-MSCs, levels of 11 cytokines and IL-2Rα were significantly increased in conditioned medium from spheroid MSCs.
Finally, to test if enhanced expression of these factors reflects altered immunomodulating activities, we assessed the effect of 2D-MSC-MVs and 3D-MSC-MVs on CD14+ cell chemoattraction.
Compared to 2D-MSC-MVs, 3D-MSC-MVs significantly decreased the chemotactic index of CD14+ cells.
Our results suggest that spheroid culture conditions improve the ability of MSCs to selectively secrete signal factors.
Moreover, 3D-MSC-MVs also possessed an enhanced capability to promote signal factors secretion compared to 2D-MSC-MVs and may possess enhanced immunomodulating activities and might be a better regenerative therapy for retinal degenerative diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xie, Lili& Mao, Mao& Zhou, Liang& Zhang, Lusi& Jiang, Bing. 2017. Signal Factors Secreted by 2D and Spheroid Mesenchymal Stem Cells and by Cocultures of Mesenchymal Stem Cells Derived Microvesicles and Retinal Photoreceptor Neurons. Stem Cells International،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1201035
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xie, Lili…[et al.]. Signal Factors Secreted by 2D and Spheroid Mesenchymal Stem Cells and by Cocultures of Mesenchymal Stem Cells Derived Microvesicles and Retinal Photoreceptor Neurons. Stem Cells International No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1201035
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xie, Lili& Mao, Mao& Zhou, Liang& Zhang, Lusi& Jiang, Bing. Signal Factors Secreted by 2D and Spheroid Mesenchymal Stem Cells and by Cocultures of Mesenchymal Stem Cells Derived Microvesicles and Retinal Photoreceptor Neurons. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1201035
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1201035
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر