Signal Factors Secreted by 2D and Spheroid Mesenchymal Stem Cells and by Cocultures of Mesenchymal Stem Cells Derived Microvesicles and Retinal Photoreceptor Neurons
Joint Authors
Jiang, Bing
Xie, Lili
Mao, Mao
Zhou, Liang
Zhang, Lusi
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-18
Country of Publication
Egypt
No. of Pages
13
Abstract EN
We aim to identify levels of signal factors secreted by MSCs cultured in 2D monolayers (2D-MSCs), spheroids (spheroids MSCs), and cocultures of microvesicles (MVs) derived from 2D-MSCs or spheroid MSCs and retinal photoreceptor neurons.
We seeded 2D-MSCs, spheroid MSCs, and cells derived from spheroids MSCs at equal numbers.
MVs isolated from all 3 culture conditions were incubated with 661W cells.
Levels of 51 signal factors in conditioned medium from those cultured conditions were quantified with bead-based assay.
We found that IL-8, IL-6, and GROα were the top three most abundant signal factors.
Moreover, compared to 2D-MSCs, levels of 11 cytokines and IL-2Rα were significantly increased in conditioned medium from spheroid MSCs.
Finally, to test if enhanced expression of these factors reflects altered immunomodulating activities, we assessed the effect of 2D-MSC-MVs and 3D-MSC-MVs on CD14+ cell chemoattraction.
Compared to 2D-MSC-MVs, 3D-MSC-MVs significantly decreased the chemotactic index of CD14+ cells.
Our results suggest that spheroid culture conditions improve the ability of MSCs to selectively secrete signal factors.
Moreover, 3D-MSC-MVs also possessed an enhanced capability to promote signal factors secretion compared to 2D-MSC-MVs and may possess enhanced immunomodulating activities and might be a better regenerative therapy for retinal degenerative diseases.
American Psychological Association (APA)
Xie, Lili& Mao, Mao& Zhou, Liang& Zhang, Lusi& Jiang, Bing. 2017. Signal Factors Secreted by 2D and Spheroid Mesenchymal Stem Cells and by Cocultures of Mesenchymal Stem Cells Derived Microvesicles and Retinal Photoreceptor Neurons. Stem Cells International،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1201035
Modern Language Association (MLA)
Xie, Lili…[et al.]. Signal Factors Secreted by 2D and Spheroid Mesenchymal Stem Cells and by Cocultures of Mesenchymal Stem Cells Derived Microvesicles and Retinal Photoreceptor Neurons. Stem Cells International No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1201035
American Medical Association (AMA)
Xie, Lili& Mao, Mao& Zhou, Liang& Zhang, Lusi& Jiang, Bing. Signal Factors Secreted by 2D and Spheroid Mesenchymal Stem Cells and by Cocultures of Mesenchymal Stem Cells Derived Microvesicles and Retinal Photoreceptor Neurons. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1201035
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1201035