High Sensitivity of Human Adipose Stem Cells to Differentiate into Myofibroblasts in the Presence of C. aspersa Egg Extract
المؤلفون المشاركون
Alvarez-Mon, Melchor
Ortega, Miguel A
Buján, Julia
García-Honduvilla, Natalio
Cifuentes, Alberto
Delgado, Arancha
González, Salvador
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-12-27
دولة النشر
مصر
عدد الصفحات
9
الملخص EN
Introduction.
Regeneration therapy using adipose-derived stem cells (ADSC) has been proposed in the treatment of skin aging.
Myofibroblast plays a relevant role in the organization of the extracellular matrix of the damaged skin.
A natural extract was derived from the eggs of the mollusk Cryptomphalus aspersa (e-CAF) that seems to play a role on skin repair.
We have investigated the potential effects of e-CAF in the differentiation of ADSC.
Materials and methods.
ADSC were cultured in the absence or presence of e-CAF (50 and 200 μg/mL) for 24 hours and 7 days.
Real-time cell assay, morphological, immunofluorescence, and RT-PCR techniques were used to evaluate the cell culture and expression of αSMA, collagen I, and tropoelastin.
Results.
e-CAF induced significant reduction in the rate of growth of ADSC from 24 hours to 7 days of culture.
e-CAF also induced bigger sizes, higher levels of cytoplasmic refringence and complexity, and a more polyhedral morphological changes in the cultured ADSC.
The protein and mRNA expression of αSMA was significantly increased in e-CAF-cultured ADSC.
Conclusion.
e-CAF promotes ADSC differentiation to myofibroblasts and should be considered as a potential agent for the prevention and treatment of skin aging.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
García-Honduvilla, Natalio& Cifuentes, Alberto& Ortega, Miguel A& Delgado, Arancha& González, Salvador& Buján, Julia…[et al.]. 2017. High Sensitivity of Human Adipose Stem Cells to Differentiate into Myofibroblasts in the Presence of C. aspersa Egg Extract. Stem Cells International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1202334
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
García-Honduvilla, Natalio…[et al.]. High Sensitivity of Human Adipose Stem Cells to Differentiate into Myofibroblasts in the Presence of C. aspersa Egg Extract. Stem Cells International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1202334
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
García-Honduvilla, Natalio& Cifuentes, Alberto& Ortega, Miguel A& Delgado, Arancha& González, Salvador& Buján, Julia…[et al.]. High Sensitivity of Human Adipose Stem Cells to Differentiate into Myofibroblasts in the Presence of C. aspersa Egg Extract. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1202334
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1202334
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر