High Sensitivity of Human Adipose Stem Cells to Differentiate into Myofibroblasts in the Presence of C. aspersa Egg Extract
Joint Authors
Alvarez-Mon, Melchor
Ortega, Miguel A
Buján, Julia
García-Honduvilla, Natalio
Cifuentes, Alberto
Delgado, Arancha
González, Salvador
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-27
Country of Publication
Egypt
No. of Pages
9
Abstract EN
Introduction.
Regeneration therapy using adipose-derived stem cells (ADSC) has been proposed in the treatment of skin aging.
Myofibroblast plays a relevant role in the organization of the extracellular matrix of the damaged skin.
A natural extract was derived from the eggs of the mollusk Cryptomphalus aspersa (e-CAF) that seems to play a role on skin repair.
We have investigated the potential effects of e-CAF in the differentiation of ADSC.
Materials and methods.
ADSC were cultured in the absence or presence of e-CAF (50 and 200 μg/mL) for 24 hours and 7 days.
Real-time cell assay, morphological, immunofluorescence, and RT-PCR techniques were used to evaluate the cell culture and expression of αSMA, collagen I, and tropoelastin.
Results.
e-CAF induced significant reduction in the rate of growth of ADSC from 24 hours to 7 days of culture.
e-CAF also induced bigger sizes, higher levels of cytoplasmic refringence and complexity, and a more polyhedral morphological changes in the cultured ADSC.
The protein and mRNA expression of αSMA was significantly increased in e-CAF-cultured ADSC.
Conclusion.
e-CAF promotes ADSC differentiation to myofibroblasts and should be considered as a potential agent for the prevention and treatment of skin aging.
American Psychological Association (APA)
García-Honduvilla, Natalio& Cifuentes, Alberto& Ortega, Miguel A& Delgado, Arancha& González, Salvador& Buján, Julia…[et al.]. 2017. High Sensitivity of Human Adipose Stem Cells to Differentiate into Myofibroblasts in the Presence of C. aspersa Egg Extract. Stem Cells International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1202334
Modern Language Association (MLA)
García-Honduvilla, Natalio…[et al.]. High Sensitivity of Human Adipose Stem Cells to Differentiate into Myofibroblasts in the Presence of C. aspersa Egg Extract. Stem Cells International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1202334
American Medical Association (AMA)
García-Honduvilla, Natalio& Cifuentes, Alberto& Ortega, Miguel A& Delgado, Arancha& González, Salvador& Buján, Julia…[et al.]. High Sensitivity of Human Adipose Stem Cells to Differentiate into Myofibroblasts in the Presence of C. aspersa Egg Extract. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1202334
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202334