Polysaccharide Hydrogels Support the Long-Term Viability of Encapsulated Human Mesenchymal Stem Cells and Their Ability to Secrete Immunomodulatory Factors
المؤلفون المشاركون
Weiss, Pierre
Guicheux, Jérôme
Hached, Fahd
Vinatier, Claire
Pinta, Pierre-Gabriel
Hulin, Philippe
Le Visage, Catherine
Billon-Chabaud, Aurélie
Grimandi, Gaël
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-10-11
دولة النشر
مصر
عدد الصفحات
11
الملخص EN
While therapeutically interesting, the injection of MSCs suffers major limitations including cell death upon injection and a massive leakage outside the injection site.
We proposed to entrap MSCs within spherical particles derived from alginate, as a control, or from silanized hydroxypropyl methylcellulose (Si-HPMC).
We developed water in an oil dispersion method to produce small Si-HPMC particles with an average size of about 68 μm.
We evidenced a faster diffusion of fluorescein isothiocyanate-dextran in Si-HPMC particles than in alginate ones.
Human adipose-derived MSCs (hASC) were encapsulated either in alginate or in Si-HPMC, and the cellularized particles were cultured for up to 1 month.
Both alginate and Si-HPMC particles supported cell survival, and the average number of encapsulated hASC per alginate and Si-HPMC particle (7102 and 5100, resp.) did not significantly change.
The stimulation of encapsulated hASC with proinflammatory cytokines resulted in the production of IDO, PGE2, and HGF whose concentration was always higher when cells were encapsulated in Si-HPMC particles than in alginate ones.
We have demonstrated that Si-HPMC and alginate particles support hASC viability and the maintenance of their ability to secrete therapeutic factors.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hached, Fahd& Vinatier, Claire& Pinta, Pierre-Gabriel& Hulin, Philippe& Le Visage, Catherine& Weiss, Pierre…[et al.]. 2017. Polysaccharide Hydrogels Support the Long-Term Viability of Encapsulated Human Mesenchymal Stem Cells and Their Ability to Secrete Immunomodulatory Factors. Stem Cells International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1202447
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hached, Fahd…[et al.]. Polysaccharide Hydrogels Support the Long-Term Viability of Encapsulated Human Mesenchymal Stem Cells and Their Ability to Secrete Immunomodulatory Factors. Stem Cells International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1202447
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hached, Fahd& Vinatier, Claire& Pinta, Pierre-Gabriel& Hulin, Philippe& Le Visage, Catherine& Weiss, Pierre…[et al.]. Polysaccharide Hydrogels Support the Long-Term Viability of Encapsulated Human Mesenchymal Stem Cells and Their Ability to Secrete Immunomodulatory Factors. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1202447
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1202447
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر