Polysaccharide Hydrogels Support the Long-Term Viability of Encapsulated Human Mesenchymal Stem Cells and Their Ability to Secrete Immunomodulatory Factors
Joint Authors
Weiss, Pierre
Guicheux, Jérôme
Hached, Fahd
Vinatier, Claire
Pinta, Pierre-Gabriel
Hulin, Philippe
Le Visage, Catherine
Billon-Chabaud, Aurélie
Grimandi, Gaël
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-11
Country of Publication
Egypt
No. of Pages
11
Abstract EN
While therapeutically interesting, the injection of MSCs suffers major limitations including cell death upon injection and a massive leakage outside the injection site.
We proposed to entrap MSCs within spherical particles derived from alginate, as a control, or from silanized hydroxypropyl methylcellulose (Si-HPMC).
We developed water in an oil dispersion method to produce small Si-HPMC particles with an average size of about 68 μm.
We evidenced a faster diffusion of fluorescein isothiocyanate-dextran in Si-HPMC particles than in alginate ones.
Human adipose-derived MSCs (hASC) were encapsulated either in alginate or in Si-HPMC, and the cellularized particles were cultured for up to 1 month.
Both alginate and Si-HPMC particles supported cell survival, and the average number of encapsulated hASC per alginate and Si-HPMC particle (7102 and 5100, resp.) did not significantly change.
The stimulation of encapsulated hASC with proinflammatory cytokines resulted in the production of IDO, PGE2, and HGF whose concentration was always higher when cells were encapsulated in Si-HPMC particles than in alginate ones.
We have demonstrated that Si-HPMC and alginate particles support hASC viability and the maintenance of their ability to secrete therapeutic factors.
American Psychological Association (APA)
Hached, Fahd& Vinatier, Claire& Pinta, Pierre-Gabriel& Hulin, Philippe& Le Visage, Catherine& Weiss, Pierre…[et al.]. 2017. Polysaccharide Hydrogels Support the Long-Term Viability of Encapsulated Human Mesenchymal Stem Cells and Their Ability to Secrete Immunomodulatory Factors. Stem Cells International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1202447
Modern Language Association (MLA)
Hached, Fahd…[et al.]. Polysaccharide Hydrogels Support the Long-Term Viability of Encapsulated Human Mesenchymal Stem Cells and Their Ability to Secrete Immunomodulatory Factors. Stem Cells International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1202447
American Medical Association (AMA)
Hached, Fahd& Vinatier, Claire& Pinta, Pierre-Gabriel& Hulin, Philippe& Le Visage, Catherine& Weiss, Pierre…[et al.]. Polysaccharide Hydrogels Support the Long-Term Viability of Encapsulated Human Mesenchymal Stem Cells and Their Ability to Secrete Immunomodulatory Factors. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1202447
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202447