Cyclophilin A Protects Cardiomyocytes against HypoxiaReoxygenation-Induced Apoptosis via the AKTNox2 Pathway
المؤلفون المشاركون
Chen, Rui
Cheng, Fuyu
Yuan, Wei
Cao, Mengfei
Wu, Xiuli
Yan, Jinchuan
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-04-28
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Hypoxia/reoxygenation (H/R) accelerates the process of cardiomyocyte apoptosis during ischemia-reperfusion.
Excessive reactive oxygen species (ROS) are a critical driver of oxidative stress injury.
Cyclophilin A (CyPA) is a major ROS-induced factor in atherosclerosis.
There is a positive feedback mechanism between CyPA and ROS, which enables the oxidative stress response to continue and expand.
However, it is unclear whether this positive feedback mechanism exists in cardiomyocytes.
Through western blotting and flow cytometric assays and TUNEL assay, we found that CyPA inhibited the apoptosis of H9c2 cardiomyocytes under H/R conditions.
By dihydroethidium (DHE) staining and electron spin resonance (ESR) assays, we demonstrated that CyPA reduced ROS production and suppressed O2- production dependent on reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase.
By western blotting, we showed that CyPA inhibited the expression of NADPH oxidase 2 (Nox2) protein by the AKT pathway.
Through confocal microscopy assay, we found that CyPA reduced the expression of Nox2 membrane-bound subunits.
The current study shows that a positive feedback mechanism does not exist in H9c2 cardiomyoblasts.
CyPA protects H9c2 cardiomyoblasts against H/R-induced apoptosis via the AKT/Nox2 pathway.
This could be a potential target for ischemia-reperfusion injury therapy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Cheng, Fuyu& Yuan, Wei& Cao, Mengfei& Chen, Rui& Wu, Xiuli& Yan, Jinchuan. 2019. Cyclophilin A Protects Cardiomyocytes against HypoxiaReoxygenation-Induced Apoptosis via the AKTNox2 Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1202856
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Cheng, Fuyu…[et al.]. Cyclophilin A Protects Cardiomyocytes against HypoxiaReoxygenation-Induced Apoptosis via the AKTNox2 Pathway. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1202856
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Cheng, Fuyu& Yuan, Wei& Cao, Mengfei& Chen, Rui& Wu, Xiuli& Yan, Jinchuan. Cyclophilin A Protects Cardiomyocytes against HypoxiaReoxygenation-Induced Apoptosis via the AKTNox2 Pathway. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1202856
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1202856
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر