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Cyclophilin A Protects Cardiomyocytes against HypoxiaReoxygenation-Induced Apoptosis via the AKTNox2 Pathway
Joint Authors
Chen, Rui
Cheng, Fuyu
Yuan, Wei
Cao, Mengfei
Wu, Xiuli
Yan, Jinchuan
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-28
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Hypoxia/reoxygenation (H/R) accelerates the process of cardiomyocyte apoptosis during ischemia-reperfusion.
Excessive reactive oxygen species (ROS) are a critical driver of oxidative stress injury.
Cyclophilin A (CyPA) is a major ROS-induced factor in atherosclerosis.
There is a positive feedback mechanism between CyPA and ROS, which enables the oxidative stress response to continue and expand.
However, it is unclear whether this positive feedback mechanism exists in cardiomyocytes.
Through western blotting and flow cytometric assays and TUNEL assay, we found that CyPA inhibited the apoptosis of H9c2 cardiomyocytes under H/R conditions.
By dihydroethidium (DHE) staining and electron spin resonance (ESR) assays, we demonstrated that CyPA reduced ROS production and suppressed O2- production dependent on reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase.
By western blotting, we showed that CyPA inhibited the expression of NADPH oxidase 2 (Nox2) protein by the AKT pathway.
Through confocal microscopy assay, we found that CyPA reduced the expression of Nox2 membrane-bound subunits.
The current study shows that a positive feedback mechanism does not exist in H9c2 cardiomyoblasts.
CyPA protects H9c2 cardiomyoblasts against H/R-induced apoptosis via the AKT/Nox2 pathway.
This could be a potential target for ischemia-reperfusion injury therapy.
American Psychological Association (APA)
Cheng, Fuyu& Yuan, Wei& Cao, Mengfei& Chen, Rui& Wu, Xiuli& Yan, Jinchuan. 2019. Cyclophilin A Protects Cardiomyocytes against HypoxiaReoxygenation-Induced Apoptosis via the AKTNox2 Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1202856
Modern Language Association (MLA)
Cheng, Fuyu…[et al.]. Cyclophilin A Protects Cardiomyocytes against HypoxiaReoxygenation-Induced Apoptosis via the AKTNox2 Pathway. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1202856
American Medical Association (AMA)
Cheng, Fuyu& Yuan, Wei& Cao, Mengfei& Chen, Rui& Wu, Xiuli& Yan, Jinchuan. Cyclophilin A Protects Cardiomyocytes against HypoxiaReoxygenation-Induced Apoptosis via the AKTNox2 Pathway. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1202856
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202856