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Investigation of Cytotoxicity Apoptotic and Inflammatory Responses of Biosynthesized Zinc Oxide Nanoparticles from Ocimum sanctum Linn in Human Skin Keratinocyte (Hacat) and Human Lung Epithelial (A549) Cells
المؤلفون المشاركون
Alarifi, Saud
Alkahtani, Saad
Albasher, Gadah
Almeer, Rafa
Almutairi, Bader
Alyami, Nouf M.
Almukhlafi, Hanouf
Yaseen, Khadijah N.
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-14
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Pristine and engineered metal nanoparticles are widely applied in various fields of industry, and as consequences, they are useful as well as harmful to human health and environment.
This study aimed at synthesizing the green zinc oxide nanoparticles (ZnNPs) using the leaf extract of Ocimum sanctum Linn and assessing its toxicity on human skin epidermal (HaCaT) and human lung epithelial (A549) cells.
The synthesized green ZnNPs (gZnNPs) were characterized by using dynamic light scattering (DLS) and a high-resolution transmission electron microscope.
The average size of gZnNPs obtained was 62 nm with a spherical shape.
The effects of gZnNPs on the viability of HaCaT and A549 cells were investigated using tetrazolium salt (MTT) for 24 h.
We have seen more reduction of cell viability of A549 cells in comparison to HaCaT cells.
The induction of intracellular reactive oxygen species (ROS) was measured using DCFDA assay and showed a slightly high intensity of green fluorescence in A549 than HaCaT cells.
The different oxidative stress biomarkers such as ROS generation and lipid peroxide were increased, and GSH was decreased in a dose-dependent manner.
The apoptotic and necrotic effect of gZnNPs in both cells was carried out using Annexin-V-FITC and propidium iodide staining.
More apoptotic and necrotic cells were found at a higher concentration of gZnNPs exposure.
Also, we determined the effect of gZnNPs at the molecular level by evaluating the apoptotic and inflammatory markers, in which gZnNPs downregulated Bcl2 and upregulated Bax, caspase-3, and TNF-α in HaCaT and A549 cells.
Ultimately, gZnNPs exerted toxicity and apoptosis in HaCaT and A549 cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Almutairi, Bader& Albasher, Gadah& Almeer, Rafa& Alyami, Nouf M.& Almukhlafi, Hanouf& Yaseen, Khadijah N.…[et al.]. 2020. Investigation of Cytotoxicity Apoptotic and Inflammatory Responses of Biosynthesized Zinc Oxide Nanoparticles from Ocimum sanctum Linn in Human Skin Keratinocyte (Hacat) and Human Lung Epithelial (A549) Cells. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1203835
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Almutairi, Bader…[et al.]. Investigation of Cytotoxicity Apoptotic and Inflammatory Responses of Biosynthesized Zinc Oxide Nanoparticles from Ocimum sanctum Linn in Human Skin Keratinocyte (Hacat) and Human Lung Epithelial (A549) Cells. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1203835
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Almutairi, Bader& Albasher, Gadah& Almeer, Rafa& Alyami, Nouf M.& Almukhlafi, Hanouf& Yaseen, Khadijah N.…[et al.]. Investigation of Cytotoxicity Apoptotic and Inflammatory Responses of Biosynthesized Zinc Oxide Nanoparticles from Ocimum sanctum Linn in Human Skin Keratinocyte (Hacat) and Human Lung Epithelial (A549) Cells. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1203835
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1203835
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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