![](/images/graphics-bg.png)
Investigation of Cytotoxicity Apoptotic and Inflammatory Responses of Biosynthesized Zinc Oxide Nanoparticles from Ocimum sanctum Linn in Human Skin Keratinocyte (Hacat) and Human Lung Epithelial (A549) Cells
Joint Authors
Alarifi, Saud
Alkahtani, Saad
Albasher, Gadah
Almeer, Rafa
Almutairi, Bader
Alyami, Nouf M.
Almukhlafi, Hanouf
Yaseen, Khadijah N.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Pristine and engineered metal nanoparticles are widely applied in various fields of industry, and as consequences, they are useful as well as harmful to human health and environment.
This study aimed at synthesizing the green zinc oxide nanoparticles (ZnNPs) using the leaf extract of Ocimum sanctum Linn and assessing its toxicity on human skin epidermal (HaCaT) and human lung epithelial (A549) cells.
The synthesized green ZnNPs (gZnNPs) were characterized by using dynamic light scattering (DLS) and a high-resolution transmission electron microscope.
The average size of gZnNPs obtained was 62 nm with a spherical shape.
The effects of gZnNPs on the viability of HaCaT and A549 cells were investigated using tetrazolium salt (MTT) for 24 h.
We have seen more reduction of cell viability of A549 cells in comparison to HaCaT cells.
The induction of intracellular reactive oxygen species (ROS) was measured using DCFDA assay and showed a slightly high intensity of green fluorescence in A549 than HaCaT cells.
The different oxidative stress biomarkers such as ROS generation and lipid peroxide were increased, and GSH was decreased in a dose-dependent manner.
The apoptotic and necrotic effect of gZnNPs in both cells was carried out using Annexin-V-FITC and propidium iodide staining.
More apoptotic and necrotic cells were found at a higher concentration of gZnNPs exposure.
Also, we determined the effect of gZnNPs at the molecular level by evaluating the apoptotic and inflammatory markers, in which gZnNPs downregulated Bcl2 and upregulated Bax, caspase-3, and TNF-α in HaCaT and A549 cells.
Ultimately, gZnNPs exerted toxicity and apoptosis in HaCaT and A549 cells.
American Psychological Association (APA)
Almutairi, Bader& Albasher, Gadah& Almeer, Rafa& Alyami, Nouf M.& Almukhlafi, Hanouf& Yaseen, Khadijah N.…[et al.]. 2020. Investigation of Cytotoxicity Apoptotic and Inflammatory Responses of Biosynthesized Zinc Oxide Nanoparticles from Ocimum sanctum Linn in Human Skin Keratinocyte (Hacat) and Human Lung Epithelial (A549) Cells. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1203835
Modern Language Association (MLA)
Almutairi, Bader…[et al.]. Investigation of Cytotoxicity Apoptotic and Inflammatory Responses of Biosynthesized Zinc Oxide Nanoparticles from Ocimum sanctum Linn in Human Skin Keratinocyte (Hacat) and Human Lung Epithelial (A549) Cells. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1203835
American Medical Association (AMA)
Almutairi, Bader& Albasher, Gadah& Almeer, Rafa& Alyami, Nouf M.& Almukhlafi, Hanouf& Yaseen, Khadijah N.…[et al.]. Investigation of Cytotoxicity Apoptotic and Inflammatory Responses of Biosynthesized Zinc Oxide Nanoparticles from Ocimum sanctum Linn in Human Skin Keratinocyte (Hacat) and Human Lung Epithelial (A549) Cells. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1203835
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203835