DNA Methylation of miR-122 Aggravates Oxidative Stress in Colitis Targeting SELENBP1 Partially by p65NF-κB Signaling
المؤلفون المشاركون
Tian, Ye
Bai, Jianan
Yu, Junchi
Wang, Jintian
Xue, Bingyan
He, Na
Yang, Lixia
Wang, Yipin
Wang, Yanyan
Tang, Qiyun
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-03-24
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Aberrant microRNA (miRNA) expressions contribute to the development and progression of various diseases, including Crohn’s disease (CD).
However, the accurate mechanisms of miRNAs in CD are definitely unclear.
We employed colonic tissue samples from normal volunteers and CD patients, an acute mice colitis model induced by 2,4,6-trinitro-benzene-sulfonic acid (TNBS), and a cellular oxidative stress model induced by H2O2 in HT-29 cells to determine the effects of oxidative stress on expressions of miR-122, selenium-binding protein 1 (SELENBP1, SBP1), p65 nuclear factor κB (p65NF-κB) signaling, and DNA methylation.
We found that SBP1 was mainly located on epithelial cells and was significantly increased in patients with active CD.
SBP1 was the target gene of miR-122.
miR-122 expression was downregulated while SBP1 expression was upregulated under TNBS-induced colitis or oxidative stress.
Pre-miR-122 or siRNA SBP1 (si-SBP1) treatment ameliorated acute TNBS-induced colitis and H2O2-induced oxidative stress.
Cotreatment of pre-miR-122 and si-SBP1 enhanced these effects.
Besides, pre-miR-122 and si-SBP1 obviously activated the p65NF-κB signaling by phosphorylation of IκBα.
Bisulfite sequencing of the CpG islands in the promoter region of miR-122 showed that CpG methylation was significantly increased under oxidative stress.
Treating cells with 5′-AZA which was well known as a DNA-demethylating agent significantly increased miR-122 expression.
Our results suggest that oxidative stress-induced DNA methylation of miR-122 aggravates colitis targeting SELENBP1 partially by p65NF-κB signaling and may promote the progression of CD.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bai, Jianan& Yu, Junchi& Wang, Jintian& Xue, Bingyan& He, Na& Tian, Ye…[et al.]. 2019. DNA Methylation of miR-122 Aggravates Oxidative Stress in Colitis Targeting SELENBP1 Partially by p65NF-κB Signaling. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1204072
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bai, Jianan…[et al.]. DNA Methylation of miR-122 Aggravates Oxidative Stress in Colitis Targeting SELENBP1 Partially by p65NF-κB Signaling. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1204072
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bai, Jianan& Yu, Junchi& Wang, Jintian& Xue, Bingyan& He, Na& Tian, Ye…[et al.]. DNA Methylation of miR-122 Aggravates Oxidative Stress in Colitis Targeting SELENBP1 Partially by p65NF-κB Signaling. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1204072
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1204072
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر