DNA Methylation of miR-122 Aggravates Oxidative Stress in Colitis Targeting SELENBP1 Partially by p65NF-κB Signaling
Joint Authors
Tian, Ye
Bai, Jianan
Yu, Junchi
Wang, Jintian
Xue, Bingyan
He, Na
Yang, Lixia
Wang, Yipin
Wang, Yanyan
Tang, Qiyun
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-24
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Aberrant microRNA (miRNA) expressions contribute to the development and progression of various diseases, including Crohn’s disease (CD).
However, the accurate mechanisms of miRNAs in CD are definitely unclear.
We employed colonic tissue samples from normal volunteers and CD patients, an acute mice colitis model induced by 2,4,6-trinitro-benzene-sulfonic acid (TNBS), and a cellular oxidative stress model induced by H2O2 in HT-29 cells to determine the effects of oxidative stress on expressions of miR-122, selenium-binding protein 1 (SELENBP1, SBP1), p65 nuclear factor κB (p65NF-κB) signaling, and DNA methylation.
We found that SBP1 was mainly located on epithelial cells and was significantly increased in patients with active CD.
SBP1 was the target gene of miR-122.
miR-122 expression was downregulated while SBP1 expression was upregulated under TNBS-induced colitis or oxidative stress.
Pre-miR-122 or siRNA SBP1 (si-SBP1) treatment ameliorated acute TNBS-induced colitis and H2O2-induced oxidative stress.
Cotreatment of pre-miR-122 and si-SBP1 enhanced these effects.
Besides, pre-miR-122 and si-SBP1 obviously activated the p65NF-κB signaling by phosphorylation of IκBα.
Bisulfite sequencing of the CpG islands in the promoter region of miR-122 showed that CpG methylation was significantly increased under oxidative stress.
Treating cells with 5′-AZA which was well known as a DNA-demethylating agent significantly increased miR-122 expression.
Our results suggest that oxidative stress-induced DNA methylation of miR-122 aggravates colitis targeting SELENBP1 partially by p65NF-κB signaling and may promote the progression of CD.
American Psychological Association (APA)
Bai, Jianan& Yu, Junchi& Wang, Jintian& Xue, Bingyan& He, Na& Tian, Ye…[et al.]. 2019. DNA Methylation of miR-122 Aggravates Oxidative Stress in Colitis Targeting SELENBP1 Partially by p65NF-κB Signaling. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1204072
Modern Language Association (MLA)
Bai, Jianan…[et al.]. DNA Methylation of miR-122 Aggravates Oxidative Stress in Colitis Targeting SELENBP1 Partially by p65NF-κB Signaling. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1204072
American Medical Association (AMA)
Bai, Jianan& Yu, Junchi& Wang, Jintian& Xue, Bingyan& He, Na& Tian, Ye…[et al.]. DNA Methylation of miR-122 Aggravates Oxidative Stress in Colitis Targeting SELENBP1 Partially by p65NF-κB Signaling. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1204072
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204072