ROS-Induced GATA4 and GATA6 Downregulation Inhibits StAR Expression in LPS-Treated Porcine Granulosa-Lutein Cells

المؤلفون المشاركون

Qu, Xiaolu
Yan, Leyan
Guo, Rihong
Li, Hui
Shi, Zhendan

المصدر

Oxidative Medicine and Cellular Longevity

العدد

المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-14، 14ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2019-04-22

دولة النشر

مصر

عدد الصفحات

14

التخصصات الرئيسية

الأحياء

الملخص EN

LPS is a major endotoxin produced by gram-negative bacteria, and exposure to it commonly occurs in animal husbandry.

Previous studies have shown that LPS infection disturbs steroidogenesis, including progesterone production, and subsequently decreases animal reproductive performance.

However, little information about the underlying mechanisms is available thus far.

In the present study, an in vitro-luteinized porcine granulosa cell model was used to study the underlying molecular mechanisms of LPS treatment.

We found that LPS significantly inhibits progesterone production and downregulates the expressions of progesterone synthesis-associated genes (StAR, CYP11A1, and 3β-HSD).

Furthermore, the levels of ROS were significantly increased in an LPS dose-dependent manner.

Moreover, transcriptional factors GATA4 and GATA6, but not NR5A1, were significantly downregulated.

Elimination of LPS-stimulated ROS by melatonin or vitamin C could restore the expressions of GATA4, GATA6, and StAR.

In parallel, StAR expression was also inhibited by the knockdown of GATA4 and GATA6.

Based on these data, we conclude that LPS impairs StAR expression via the ROS-induced downregulation of GATA4 and GATA6.

Collectively, these findings provide new insights into the understanding of reproductive losses in animals suffering from bacterial infection and LPS exposure.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Qu, Xiaolu& Yan, Leyan& Guo, Rihong& Li, Hui& Shi, Zhendan. 2019. ROS-Induced GATA4 and GATA6 Downregulation Inhibits StAR Expression in LPS-Treated Porcine Granulosa-Lutein Cells. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1204158

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Qu, Xiaolu…[et al.]. ROS-Induced GATA4 and GATA6 Downregulation Inhibits StAR Expression in LPS-Treated Porcine Granulosa-Lutein Cells. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1204158

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Qu, Xiaolu& Yan, Leyan& Guo, Rihong& Li, Hui& Shi, Zhendan. ROS-Induced GATA4 and GATA6 Downregulation Inhibits StAR Expression in LPS-Treated Porcine Granulosa-Lutein Cells. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1204158

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1204158