ROS-Induced GATA4 and GATA6 Downregulation Inhibits StAR Expression in LPS-Treated Porcine Granulosa-Lutein Cells
المؤلفون المشاركون
Qu, Xiaolu
Yan, Leyan
Guo, Rihong
Li, Hui
Shi, Zhendan
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-04-22
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
LPS is a major endotoxin produced by gram-negative bacteria, and exposure to it commonly occurs in animal husbandry.
Previous studies have shown that LPS infection disturbs steroidogenesis, including progesterone production, and subsequently decreases animal reproductive performance.
However, little information about the underlying mechanisms is available thus far.
In the present study, an in vitro-luteinized porcine granulosa cell model was used to study the underlying molecular mechanisms of LPS treatment.
We found that LPS significantly inhibits progesterone production and downregulates the expressions of progesterone synthesis-associated genes (StAR, CYP11A1, and 3β-HSD).
Furthermore, the levels of ROS were significantly increased in an LPS dose-dependent manner.
Moreover, transcriptional factors GATA4 and GATA6, but not NR5A1, were significantly downregulated.
Elimination of LPS-stimulated ROS by melatonin or vitamin C could restore the expressions of GATA4, GATA6, and StAR.
In parallel, StAR expression was also inhibited by the knockdown of GATA4 and GATA6.
Based on these data, we conclude that LPS impairs StAR expression via the ROS-induced downregulation of GATA4 and GATA6.
Collectively, these findings provide new insights into the understanding of reproductive losses in animals suffering from bacterial infection and LPS exposure.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Qu, Xiaolu& Yan, Leyan& Guo, Rihong& Li, Hui& Shi, Zhendan. 2019. ROS-Induced GATA4 and GATA6 Downregulation Inhibits StAR Expression in LPS-Treated Porcine Granulosa-Lutein Cells. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1204158
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Qu, Xiaolu…[et al.]. ROS-Induced GATA4 and GATA6 Downregulation Inhibits StAR Expression in LPS-Treated Porcine Granulosa-Lutein Cells. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1204158
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Qu, Xiaolu& Yan, Leyan& Guo, Rihong& Li, Hui& Shi, Zhendan. ROS-Induced GATA4 and GATA6 Downregulation Inhibits StAR Expression in LPS-Treated Porcine Granulosa-Lutein Cells. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1204158
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1204158
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر