ROS-Induced GATA4 and GATA6 Downregulation Inhibits StAR Expression in LPS-Treated Porcine Granulosa-Lutein Cells

Joint Authors

Qu, Xiaolu
Yan, Leyan
Guo, Rihong
Li, Hui
Shi, Zhendan

Source

Oxidative Medicine and Cellular Longevity

Issue

Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2019-04-22

Country of Publication

Egypt

No. of Pages

14

Main Subjects

Biology

Abstract EN

LPS is a major endotoxin produced by gram-negative bacteria, and exposure to it commonly occurs in animal husbandry.

Previous studies have shown that LPS infection disturbs steroidogenesis, including progesterone production, and subsequently decreases animal reproductive performance.

However, little information about the underlying mechanisms is available thus far.

In the present study, an in vitro-luteinized porcine granulosa cell model was used to study the underlying molecular mechanisms of LPS treatment.

We found that LPS significantly inhibits progesterone production and downregulates the expressions of progesterone synthesis-associated genes (StAR, CYP11A1, and 3β-HSD).

Furthermore, the levels of ROS were significantly increased in an LPS dose-dependent manner.

Moreover, transcriptional factors GATA4 and GATA6, but not NR5A1, were significantly downregulated.

Elimination of LPS-stimulated ROS by melatonin or vitamin C could restore the expressions of GATA4, GATA6, and StAR.

In parallel, StAR expression was also inhibited by the knockdown of GATA4 and GATA6.

Based on these data, we conclude that LPS impairs StAR expression via the ROS-induced downregulation of GATA4 and GATA6.

Collectively, these findings provide new insights into the understanding of reproductive losses in animals suffering from bacterial infection and LPS exposure.

American Psychological Association (APA)

Qu, Xiaolu& Yan, Leyan& Guo, Rihong& Li, Hui& Shi, Zhendan. 2019. ROS-Induced GATA4 and GATA6 Downregulation Inhibits StAR Expression in LPS-Treated Porcine Granulosa-Lutein Cells. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1204158

Modern Language Association (MLA)

Qu, Xiaolu…[et al.]. ROS-Induced GATA4 and GATA6 Downregulation Inhibits StAR Expression in LPS-Treated Porcine Granulosa-Lutein Cells. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1204158

American Medical Association (AMA)

Qu, Xiaolu& Yan, Leyan& Guo, Rihong& Li, Hui& Shi, Zhendan. ROS-Induced GATA4 and GATA6 Downregulation Inhibits StAR Expression in LPS-Treated Porcine Granulosa-Lutein Cells. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1204158

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1204158