Activation of TGR5 Partially Alleviates High Glucose-Induced Cardiomyocyte Injury by Inhibition of Inflammatory Responses and Oxidative Stress
المؤلفون المشاركون
Deng, Li
Feng, Jian
Zhong, Yi
Li, Jiafu
Chen, Xuxin
Wen, Xing
Cai, Ying
Fan, Zhongcai
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-21
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
High glucose- (HG-) induced cardiomyocyte injury is the leading cause of diabetic cardiomyopathy, which is associated with the induction of inflammatory responses and oxidative stress.
TGR5 plays an important role in the regulation of glucose metabolism.
However, whether TGR5 has cardioprotective effects against HG-induced cardiomyocyte injury is unknown.
Neonatal mouse cardiomyocytes were isolated and incubated in a HG medium.
Protein and mRNA expression was detected by western blotting and RT-PCR, respectively.
Cell apoptosis was determined by Hoechst 33342 staining and flow cytometry.
After treatment of cells with HG, TGR5-selective agonist INT-777 reduced the increase in expression of proinflammatory cytokines and NF-κB, whereas pretreatment of cells with TGR5 shRNA significantly reduced the inhibitory effects of INT-777.
We also found that INT-777 increased the protein expression of Nrf2 and HO-1.
In the presence of TGR5 shRNA, the expression of Nrf2 and HO-1 was reduced, indicating that TGR5 may exert an antioxidant effect partially through the Nrf2/HO-1 pathway.
Furthermore, INT-777 treatment inhibited HG-induced ROS production and apoptosis that were attenuated in the presence of TGR5 shRNA or ZnPP (HO-1 inhibitor).
Activation of TGR5 has cardioprotective effects against HG-induced cardiomyocyte injury and could be a pharmacological target for the treatment of diabetic cardiomyopathy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Deng, Li& Chen, Xuxin& Zhong, Yi& Wen, Xing& Cai, Ying& Li, Jiafu…[et al.]. 2019. Activation of TGR5 Partially Alleviates High Glucose-Induced Cardiomyocyte Injury by Inhibition of Inflammatory Responses and Oxidative Stress. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1204572
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Deng, Li…[et al.]. Activation of TGR5 Partially Alleviates High Glucose-Induced Cardiomyocyte Injury by Inhibition of Inflammatory Responses and Oxidative Stress. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1204572
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Deng, Li& Chen, Xuxin& Zhong, Yi& Wen, Xing& Cai, Ying& Li, Jiafu…[et al.]. Activation of TGR5 Partially Alleviates High Glucose-Induced Cardiomyocyte Injury by Inhibition of Inflammatory Responses and Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1204572
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1204572
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر