Activation of TGR5 Partially Alleviates High Glucose-Induced Cardiomyocyte Injury by Inhibition of Inflammatory Responses and Oxidative Stress
Joint Authors
Deng, Li
Feng, Jian
Zhong, Yi
Li, Jiafu
Chen, Xuxin
Wen, Xing
Cai, Ying
Fan, Zhongcai
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-21
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
High glucose- (HG-) induced cardiomyocyte injury is the leading cause of diabetic cardiomyopathy, which is associated with the induction of inflammatory responses and oxidative stress.
TGR5 plays an important role in the regulation of glucose metabolism.
However, whether TGR5 has cardioprotective effects against HG-induced cardiomyocyte injury is unknown.
Neonatal mouse cardiomyocytes were isolated and incubated in a HG medium.
Protein and mRNA expression was detected by western blotting and RT-PCR, respectively.
Cell apoptosis was determined by Hoechst 33342 staining and flow cytometry.
After treatment of cells with HG, TGR5-selective agonist INT-777 reduced the increase in expression of proinflammatory cytokines and NF-κB, whereas pretreatment of cells with TGR5 shRNA significantly reduced the inhibitory effects of INT-777.
We also found that INT-777 increased the protein expression of Nrf2 and HO-1.
In the presence of TGR5 shRNA, the expression of Nrf2 and HO-1 was reduced, indicating that TGR5 may exert an antioxidant effect partially through the Nrf2/HO-1 pathway.
Furthermore, INT-777 treatment inhibited HG-induced ROS production and apoptosis that were attenuated in the presence of TGR5 shRNA or ZnPP (HO-1 inhibitor).
Activation of TGR5 has cardioprotective effects against HG-induced cardiomyocyte injury and could be a pharmacological target for the treatment of diabetic cardiomyopathy.
American Psychological Association (APA)
Deng, Li& Chen, Xuxin& Zhong, Yi& Wen, Xing& Cai, Ying& Li, Jiafu…[et al.]. 2019. Activation of TGR5 Partially Alleviates High Glucose-Induced Cardiomyocyte Injury by Inhibition of Inflammatory Responses and Oxidative Stress. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1204572
Modern Language Association (MLA)
Deng, Li…[et al.]. Activation of TGR5 Partially Alleviates High Glucose-Induced Cardiomyocyte Injury by Inhibition of Inflammatory Responses and Oxidative Stress. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1204572
American Medical Association (AMA)
Deng, Li& Chen, Xuxin& Zhong, Yi& Wen, Xing& Cai, Ying& Li, Jiafu…[et al.]. Activation of TGR5 Partially Alleviates High Glucose-Induced Cardiomyocyte Injury by Inhibition of Inflammatory Responses and Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1204572
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1204572