Aspirin-Mediated Attenuation of Intervertebral Disc Degeneration by Ameliorating Reactive Oxygen Species In Vivo and In Vitro
المؤلفون المشاركون
Liu, Yu
Shen, Jining
Yu, Binqing
Bai, Jiaxiang
Lin, Jiayi
Guo, Xiaobin
Sun, Houyi
Geng, Dechun
Wu, Xiexing
Chen, Zhanghuan
Yang, Huilin
Mao, Haiqing
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-20، 20ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-06
دولة النشر
مصر
عدد الصفحات
20
التخصصات الرئيسية
الملخص EN
Intervertebral disc (IVD) degeneration (IDD) is a major cause of low back pain.
The pathogenesis of IDD is associated with the disturbance of reactive oxygen species (ROS) equilibrium, inflammation, and matrix loss.
Aspirin is a nonsteroidal anti-inflammatory drug that effectively inhibits inflammation and oxidative stress and has been widely used for the treatment of back pain.
Therefore, we hypothesize that aspirin reverses the IDD process via antioxidative and anti-inflammatory effects on the AMPK signaling pathway.
In vitro, aspirin diminished cellular oxygen free radicals (ROS, nitric oxide (NO)) and inflammatory cytokines (interleukin- (IL-) 1β and IL-6 and tumor necrosis factor alpha (TNF-α)) induced by lipopolysaccharides (LPS) in nucleus pulposus cells (NPCs).
We found that aspirin preserved the extracellular matrix (ECM) content of collagen type II (COL2) and aggrecan while inhibiting the expression of matrix-degenerating enzymes, including matrix metalloproteinase 3 and 13 (MMP-3 and MMP-13) and A disintegrin and metalloproteinase with thrombospondin motifs 4 and 5 (ADAMTS-4, ADAMTS-5).
Aspirin significantly promoted the ratios of p-AMPK to AMPK and p-ACC to ACC expression in NPCs.
Furthermore, pretreatment with the AMPK inhibitor compound C abrogated the antioxidant effects of aspirin.
In vivo, an IDD model was established in Sprague-Dawley rats via percutaneous disc puncture with the 20-gauge needle on levels 8-9 and 9-10 of the coccygeal vertebrae.
Imaging assessment showed that after aspirin treatment, improvements in disc height index (DHI) ranged from 1.22-fold to 1.54-fold and nucleus pulposus signal strength improved from 1.26-fold to 1.33-fold.
Histological analysis showed that aspirin treatment prevented the loss of COL2 and decreased MMP-3 and MMP-13, inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), IL-1β, and TNF-α expression in the IVD tissues.
These results suggest that treatment with aspirin could reverse the IDD process via the AMPK signaling pathway, which provides new insights into the potential clinical applications of aspirin, particularly for IDD treatment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Yu& Lin, Jiayi& Wu, Xiexing& Guo, Xiaobin& Sun, Houyi& Yu, Binqing…[et al.]. 2019. Aspirin-Mediated Attenuation of Intervertebral Disc Degeneration by Ameliorating Reactive Oxygen Species In Vivo and In Vitro. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-20.
https://search.emarefa.net/detail/BIM-1205027
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Yu…[et al.]. Aspirin-Mediated Attenuation of Intervertebral Disc Degeneration by Ameliorating Reactive Oxygen Species In Vivo and In Vitro. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-20.
https://search.emarefa.net/detail/BIM-1205027
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Yu& Lin, Jiayi& Wu, Xiexing& Guo, Xiaobin& Sun, Houyi& Yu, Binqing…[et al.]. Aspirin-Mediated Attenuation of Intervertebral Disc Degeneration by Ameliorating Reactive Oxygen Species In Vivo and In Vitro. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-20.
https://search.emarefa.net/detail/BIM-1205027
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1205027
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر