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Aspirin-Mediated Attenuation of Intervertebral Disc Degeneration by Ameliorating Reactive Oxygen Species In Vivo and In Vitro
Joint Authors
Liu, Yu
Shen, Jining
Yu, Binqing
Bai, Jiaxiang
Lin, Jiayi
Guo, Xiaobin
Sun, Houyi
Geng, Dechun
Wu, Xiexing
Chen, Zhanghuan
Yang, Huilin
Mao, Haiqing
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-20, 20 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-06
Country of Publication
Egypt
No. of Pages
20
Main Subjects
Abstract EN
Intervertebral disc (IVD) degeneration (IDD) is a major cause of low back pain.
The pathogenesis of IDD is associated with the disturbance of reactive oxygen species (ROS) equilibrium, inflammation, and matrix loss.
Aspirin is a nonsteroidal anti-inflammatory drug that effectively inhibits inflammation and oxidative stress and has been widely used for the treatment of back pain.
Therefore, we hypothesize that aspirin reverses the IDD process via antioxidative and anti-inflammatory effects on the AMPK signaling pathway.
In vitro, aspirin diminished cellular oxygen free radicals (ROS, nitric oxide (NO)) and inflammatory cytokines (interleukin- (IL-) 1β and IL-6 and tumor necrosis factor alpha (TNF-α)) induced by lipopolysaccharides (LPS) in nucleus pulposus cells (NPCs).
We found that aspirin preserved the extracellular matrix (ECM) content of collagen type II (COL2) and aggrecan while inhibiting the expression of matrix-degenerating enzymes, including matrix metalloproteinase 3 and 13 (MMP-3 and MMP-13) and A disintegrin and metalloproteinase with thrombospondin motifs 4 and 5 (ADAMTS-4, ADAMTS-5).
Aspirin significantly promoted the ratios of p-AMPK to AMPK and p-ACC to ACC expression in NPCs.
Furthermore, pretreatment with the AMPK inhibitor compound C abrogated the antioxidant effects of aspirin.
In vivo, an IDD model was established in Sprague-Dawley rats via percutaneous disc puncture with the 20-gauge needle on levels 8-9 and 9-10 of the coccygeal vertebrae.
Imaging assessment showed that after aspirin treatment, improvements in disc height index (DHI) ranged from 1.22-fold to 1.54-fold and nucleus pulposus signal strength improved from 1.26-fold to 1.33-fold.
Histological analysis showed that aspirin treatment prevented the loss of COL2 and decreased MMP-3 and MMP-13, inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), IL-1β, and TNF-α expression in the IVD tissues.
These results suggest that treatment with aspirin could reverse the IDD process via the AMPK signaling pathway, which provides new insights into the potential clinical applications of aspirin, particularly for IDD treatment.
American Psychological Association (APA)
Liu, Yu& Lin, Jiayi& Wu, Xiexing& Guo, Xiaobin& Sun, Houyi& Yu, Binqing…[et al.]. 2019. Aspirin-Mediated Attenuation of Intervertebral Disc Degeneration by Ameliorating Reactive Oxygen Species In Vivo and In Vitro. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-20.
https://search.emarefa.net/detail/BIM-1205027
Modern Language Association (MLA)
Liu, Yu…[et al.]. Aspirin-Mediated Attenuation of Intervertebral Disc Degeneration by Ameliorating Reactive Oxygen Species In Vivo and In Vitro. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-20.
https://search.emarefa.net/detail/BIM-1205027
American Medical Association (AMA)
Liu, Yu& Lin, Jiayi& Wu, Xiexing& Guo, Xiaobin& Sun, Houyi& Yu, Binqing…[et al.]. Aspirin-Mediated Attenuation of Intervertebral Disc Degeneration by Ameliorating Reactive Oxygen Species In Vivo and In Vitro. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-20.
https://search.emarefa.net/detail/BIM-1205027
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205027