EGCG Attenuates Renal Damage via Reversing Klotho Hypermethylation in Diabetic dbdb Mice and HK-2 Cells
المؤلفون المشاركون
Guo, Li Li
Jin, Hui Min
Yang, Xiu Hong
Zhang, Bao Long
Zhang, Xiao Meng
Tong, Jin Dong
Gu, Yan Hong
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-27
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
To explore whether epigallocatechin-3-gallate (EGCG) improves renal damage in diabetic db/db mice and high-glucose- (HG-) induced injury in HK-2 cells by regulating the level of Klotho gene promoter methylation.
Western blotting was used to detect the protein expression levels of DNA methyltransferase 1 (DNMT1), DNMT3a, DNMT3b, transforming growth factor-β1 (TGF-β1), α-smooth muscle actin (α-SMA), and Klotho.
The methylation level of the Klotho gene promoter was detected by pyrosequencing.
Chromatin immunoprecipitation was used to detect the binding of the Klotho gene promoter to DNMT1 and DNMT3a.
The expression of oxidative stress markers (reactive oxygen species (ROS), superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), and 8-hydroxy-2′-deoxyguanosine (8-OHdG)) and inflammatory cytokines (interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α)) in kidney homogenates was also measured using ELISA.
Klotho and DNMT3b protein expression was upregulated, while DNMT1, DNMT3a, TGF-β1, and α-SMA protein expression was downregulated after EGCG treatment.
EGCG treatment also reduced the methylation level of the Klotho gene promoter as well as the binding of DNMT3a to the Klotho gene promoter.
In addition, EGCG treatment significantly decreased the levels of ROS, MDA, 8-OHdG, IL-1β, IL-6, and TNF-α and increased the levels of CAT and SOD.
Under HG conditions, EGCG regulated Klotho gene promoter methylation via DNMT3a and decreased the methylation level of the Klotho gene promoter, thereby upregulating the expression of the Klotho protein to exert its protective effect.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Xiu Hong& Zhang, Bao Long& Zhang, Xiao Meng& Tong, Jin Dong& Gu, Yan Hong& Guo, Li Li…[et al.]. 2020. EGCG Attenuates Renal Damage via Reversing Klotho Hypermethylation in Diabetic dbdb Mice and HK-2 Cells. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1205041
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Xiu Hong…[et al.]. EGCG Attenuates Renal Damage via Reversing Klotho Hypermethylation in Diabetic dbdb Mice and HK-2 Cells. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1205041
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Xiu Hong& Zhang, Bao Long& Zhang, Xiao Meng& Tong, Jin Dong& Gu, Yan Hong& Guo, Li Li…[et al.]. EGCG Attenuates Renal Damage via Reversing Klotho Hypermethylation in Diabetic dbdb Mice and HK-2 Cells. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1205041
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1205041
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر