EGCG Attenuates Renal Damage via Reversing Klotho Hypermethylation in Diabetic dbdb Mice and HK-2 Cells
Joint Authors
Guo, Li Li
Jin, Hui Min
Yang, Xiu Hong
Zhang, Bao Long
Zhang, Xiao Meng
Tong, Jin Dong
Gu, Yan Hong
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-27
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
To explore whether epigallocatechin-3-gallate (EGCG) improves renal damage in diabetic db/db mice and high-glucose- (HG-) induced injury in HK-2 cells by regulating the level of Klotho gene promoter methylation.
Western blotting was used to detect the protein expression levels of DNA methyltransferase 1 (DNMT1), DNMT3a, DNMT3b, transforming growth factor-β1 (TGF-β1), α-smooth muscle actin (α-SMA), and Klotho.
The methylation level of the Klotho gene promoter was detected by pyrosequencing.
Chromatin immunoprecipitation was used to detect the binding of the Klotho gene promoter to DNMT1 and DNMT3a.
The expression of oxidative stress markers (reactive oxygen species (ROS), superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), and 8-hydroxy-2′-deoxyguanosine (8-OHdG)) and inflammatory cytokines (interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α)) in kidney homogenates was also measured using ELISA.
Klotho and DNMT3b protein expression was upregulated, while DNMT1, DNMT3a, TGF-β1, and α-SMA protein expression was downregulated after EGCG treatment.
EGCG treatment also reduced the methylation level of the Klotho gene promoter as well as the binding of DNMT3a to the Klotho gene promoter.
In addition, EGCG treatment significantly decreased the levels of ROS, MDA, 8-OHdG, IL-1β, IL-6, and TNF-α and increased the levels of CAT and SOD.
Under HG conditions, EGCG regulated Klotho gene promoter methylation via DNMT3a and decreased the methylation level of the Klotho gene promoter, thereby upregulating the expression of the Klotho protein to exert its protective effect.
American Psychological Association (APA)
Yang, Xiu Hong& Zhang, Bao Long& Zhang, Xiao Meng& Tong, Jin Dong& Gu, Yan Hong& Guo, Li Li…[et al.]. 2020. EGCG Attenuates Renal Damage via Reversing Klotho Hypermethylation in Diabetic dbdb Mice and HK-2 Cells. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1205041
Modern Language Association (MLA)
Yang, Xiu Hong…[et al.]. EGCG Attenuates Renal Damage via Reversing Klotho Hypermethylation in Diabetic dbdb Mice and HK-2 Cells. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1205041
American Medical Association (AMA)
Yang, Xiu Hong& Zhang, Bao Long& Zhang, Xiao Meng& Tong, Jin Dong& Gu, Yan Hong& Guo, Li Li…[et al.]. EGCG Attenuates Renal Damage via Reversing Klotho Hypermethylation in Diabetic dbdb Mice and HK-2 Cells. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1205041
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205041