KLF2 Protects against Osteoarthritis by Repressing Oxidative Response through Activation of Nrf2ARE Signaling In Vitro and In Vivo
المؤلفون المشاركون
Gao, Xiang
Li, Xu
Jiang, Shuangpeng
Du, Zhangzhen
Ke, Angtin
Liang, Qingwei
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-18، 18ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-19
دولة النشر
مصر
عدد الصفحات
18
التخصصات الرئيسية
الملخص EN
Osteoarthritis (OA) is a multifactorial and inflammatory disease characterized by cartilage destruction that can cause disability among aging patients.
There is currently no effective treatment that can arrest or reverse OA progression.
Kruppel-like factor 2 (KLF2), a member of the zinc finger family, has emerged as a transcription factor involved in a wide variety of inflammatory diseases.
Here, we identified that KLF2 expression is downregulated in IL-1β-treated human chondrocytes and OA cartilage.
Genetic and pharmacological overexpression of KLF2 suppressed IL-1β-induced apoptosis and matrix degradation through the suppression of reactive oxygen species (ROS) production.
In addition, KLF2 overexpression resulted in increased expression of heme oxygenase-1 (HO-1) and NAD(P)H dehydrogenase quinone 1 (NQO1) through the enhanced nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2).
Further, Nrf2 inhibition abrogated the chondroprotective effects of KLF2.
Safranin O/fast green and TUNEL staining demonstrated that adenovirus-mediated overexpression of KLF2 in joint cartilage protects rats against experimental OA by inhibiting cartilage degradation and chondrocyte apoptosis.
Immunohistochemical staining revealed that KLF2 overexpression significantly decreases MMP13 expression caused by OA progression in vivo.
This in vitro and in vivo study is the first to investigate the antioxidative effect and mechanisms of KLF2 in OA pathogenesis.
Our results collectively provide new insights into OA pathogenesis regulated by KLF2 and a rationale for the development of effective OA intervention strategies.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gao, Xiang& Jiang, Shuangpeng& Du, Zhangzhen& Ke, Angtin& Liang, Qingwei& Li, Xu. 2019. KLF2 Protects against Osteoarthritis by Repressing Oxidative Response through Activation of Nrf2ARE Signaling In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1205773
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gao, Xiang…[et al.]. KLF2 Protects against Osteoarthritis by Repressing Oxidative Response through Activation of Nrf2ARE Signaling In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-18.
https://search.emarefa.net/detail/BIM-1205773
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gao, Xiang& Jiang, Shuangpeng& Du, Zhangzhen& Ke, Angtin& Liang, Qingwei& Li, Xu. KLF2 Protects against Osteoarthritis by Repressing Oxidative Response through Activation of Nrf2ARE Signaling In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1205773
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1205773
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر