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KLF2 Protects against Osteoarthritis by Repressing Oxidative Response through Activation of Nrf2ARE Signaling In Vitro and In Vivo
Joint Authors
Gao, Xiang
Li, Xu
Jiang, Shuangpeng
Du, Zhangzhen
Ke, Angtin
Liang, Qingwei
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-19
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Osteoarthritis (OA) is a multifactorial and inflammatory disease characterized by cartilage destruction that can cause disability among aging patients.
There is currently no effective treatment that can arrest or reverse OA progression.
Kruppel-like factor 2 (KLF2), a member of the zinc finger family, has emerged as a transcription factor involved in a wide variety of inflammatory diseases.
Here, we identified that KLF2 expression is downregulated in IL-1β-treated human chondrocytes and OA cartilage.
Genetic and pharmacological overexpression of KLF2 suppressed IL-1β-induced apoptosis and matrix degradation through the suppression of reactive oxygen species (ROS) production.
In addition, KLF2 overexpression resulted in increased expression of heme oxygenase-1 (HO-1) and NAD(P)H dehydrogenase quinone 1 (NQO1) through the enhanced nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2).
Further, Nrf2 inhibition abrogated the chondroprotective effects of KLF2.
Safranin O/fast green and TUNEL staining demonstrated that adenovirus-mediated overexpression of KLF2 in joint cartilage protects rats against experimental OA by inhibiting cartilage degradation and chondrocyte apoptosis.
Immunohistochemical staining revealed that KLF2 overexpression significantly decreases MMP13 expression caused by OA progression in vivo.
This in vitro and in vivo study is the first to investigate the antioxidative effect and mechanisms of KLF2 in OA pathogenesis.
Our results collectively provide new insights into OA pathogenesis regulated by KLF2 and a rationale for the development of effective OA intervention strategies.
American Psychological Association (APA)
Gao, Xiang& Jiang, Shuangpeng& Du, Zhangzhen& Ke, Angtin& Liang, Qingwei& Li, Xu. 2019. KLF2 Protects against Osteoarthritis by Repressing Oxidative Response through Activation of Nrf2ARE Signaling In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1205773
Modern Language Association (MLA)
Gao, Xiang…[et al.]. KLF2 Protects against Osteoarthritis by Repressing Oxidative Response through Activation of Nrf2ARE Signaling In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-18.
https://search.emarefa.net/detail/BIM-1205773
American Medical Association (AMA)
Gao, Xiang& Jiang, Shuangpeng& Du, Zhangzhen& Ke, Angtin& Liang, Qingwei& Li, Xu. KLF2 Protects against Osteoarthritis by Repressing Oxidative Response through Activation of Nrf2ARE Signaling In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1205773
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1205773