Resveratrol Inhibits MMP3 and MMP9 Expression and Secretion by Suppressing TLR4NF-κBSTAT3 Activation in Ox-LDL-Treated HUVECs
المؤلفون المشاركون
Zhao, Yanyang
Qi, Ruomei
Meng, Ling-bing
Gong, Huan
Zhang, Ming
Xue, Yun
Chen, Huilian
Chen, Beidong
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-09-10
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Aim.
Resveratrol is a natural plant polyphenol.
The present study investigated the effects of resveratrol on the Toll-like receptor 4- (TLR4-) mediated expression and secretion of matrix metalloproteinases (MMPs) in oxidized low-density lipoprotein- (ox-LDL-) treated human umbilical vein endothelial cells (HUVECs).
Methods.
Protein expression was analyzed by immunoblotting.
The secretion of MMPs was measured by an enzyme-linked immunosorbent assay.
The animal experiments were performed with and without resveratrol treatment in high-fat chow-fed mice.
Results.
Resveratrol inhibited the expression of TLR4, MMP3, and MMP9 in ox-LDL- and lipopolysaccharide- (LPS-) treated HUVECs.
Resveratrol reduced the secretion of MMP3 and MMP9 that was induced by ox-LDL and LPS.
The TLR4 inhibitor CLI-095 similarly suppressed the expression and secretion of MMP3 and MMP9 in ox-LDL- and LPS-treated HUVECs.
Resveratrol attenuated the phosphorylation of the transcription factors nuclear factor-κB (NF-κB) and signal transducer and activator of transcription 3 (STAT3) that was induced by ox-LDL and LPS.
Resveratrol recovered Sirt1 expression.
In the animal experiments, resveratrol decreased TLR4 expression in the aorta, MMP9 levels in plasma, and vascular structural changes in high-fat chow-fed mice, with no significant effect on plasma MMP3 levels.
Conclusion.
Resveratrol inhibited the TLR4-mediated expression and secretion of MMP3 and MMP9 in ox-LDL-treated HUVECs.
The mechanism of action of resveratrol may be associated with the suppression of NF-κB and STAT3 phosphorylation and restoration of Sirt1 expression.
Resveratrol exerts protective effects against vascular structural changes in high-fat chow-fed mice.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Ming& Xue, Yun& Chen, Huilian& Meng, Ling-bing& Chen, Beidong& Gong, Huan…[et al.]. 2019. Resveratrol Inhibits MMP3 and MMP9 Expression and Secretion by Suppressing TLR4NF-κBSTAT3 Activation in Ox-LDL-Treated HUVECs. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1206028
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Ming…[et al.]. Resveratrol Inhibits MMP3 and MMP9 Expression and Secretion by Suppressing TLR4NF-κBSTAT3 Activation in Ox-LDL-Treated HUVECs. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1206028
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Ming& Xue, Yun& Chen, Huilian& Meng, Ling-bing& Chen, Beidong& Gong, Huan…[et al.]. Resveratrol Inhibits MMP3 and MMP9 Expression and Secretion by Suppressing TLR4NF-κBSTAT3 Activation in Ox-LDL-Treated HUVECs. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1206028
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1206028
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر