Resveratrol Inhibits MMP3 and MMP9 Expression and Secretion by Suppressing TLR4NF-κBSTAT3 Activation in Ox-LDL-Treated HUVECs
Joint Authors
Zhao, Yanyang
Qi, Ruomei
Meng, Ling-bing
Gong, Huan
Zhang, Ming
Xue, Yun
Chen, Huilian
Chen, Beidong
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-10
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Aim.
Resveratrol is a natural plant polyphenol.
The present study investigated the effects of resveratrol on the Toll-like receptor 4- (TLR4-) mediated expression and secretion of matrix metalloproteinases (MMPs) in oxidized low-density lipoprotein- (ox-LDL-) treated human umbilical vein endothelial cells (HUVECs).
Methods.
Protein expression was analyzed by immunoblotting.
The secretion of MMPs was measured by an enzyme-linked immunosorbent assay.
The animal experiments were performed with and without resveratrol treatment in high-fat chow-fed mice.
Results.
Resveratrol inhibited the expression of TLR4, MMP3, and MMP9 in ox-LDL- and lipopolysaccharide- (LPS-) treated HUVECs.
Resveratrol reduced the secretion of MMP3 and MMP9 that was induced by ox-LDL and LPS.
The TLR4 inhibitor CLI-095 similarly suppressed the expression and secretion of MMP3 and MMP9 in ox-LDL- and LPS-treated HUVECs.
Resveratrol attenuated the phosphorylation of the transcription factors nuclear factor-κB (NF-κB) and signal transducer and activator of transcription 3 (STAT3) that was induced by ox-LDL and LPS.
Resveratrol recovered Sirt1 expression.
In the animal experiments, resveratrol decreased TLR4 expression in the aorta, MMP9 levels in plasma, and vascular structural changes in high-fat chow-fed mice, with no significant effect on plasma MMP3 levels.
Conclusion.
Resveratrol inhibited the TLR4-mediated expression and secretion of MMP3 and MMP9 in ox-LDL-treated HUVECs.
The mechanism of action of resveratrol may be associated with the suppression of NF-κB and STAT3 phosphorylation and restoration of Sirt1 expression.
Resveratrol exerts protective effects against vascular structural changes in high-fat chow-fed mice.
American Psychological Association (APA)
Zhang, Ming& Xue, Yun& Chen, Huilian& Meng, Ling-bing& Chen, Beidong& Gong, Huan…[et al.]. 2019. Resveratrol Inhibits MMP3 and MMP9 Expression and Secretion by Suppressing TLR4NF-κBSTAT3 Activation in Ox-LDL-Treated HUVECs. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1206028
Modern Language Association (MLA)
Zhang, Ming…[et al.]. Resveratrol Inhibits MMP3 and MMP9 Expression and Secretion by Suppressing TLR4NF-κBSTAT3 Activation in Ox-LDL-Treated HUVECs. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1206028
American Medical Association (AMA)
Zhang, Ming& Xue, Yun& Chen, Huilian& Meng, Ling-bing& Chen, Beidong& Gong, Huan…[et al.]. Resveratrol Inhibits MMP3 and MMP9 Expression and Secretion by Suppressing TLR4NF-κBSTAT3 Activation in Ox-LDL-Treated HUVECs. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1206028
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1206028