Endothelin-1 Downregulates Sulfur DioxideAspartate Aminotransferase Pathway via Reactive Oxygen Species to Promote the Proliferation and Migration of Vascular Smooth Muscle Cells

المؤلفون المشاركون

Junbao, D.
Huang, Yaqian
Sun, Yan
Tang, Chaoshu
Jin, Hongfang
Chen, Selena
Tian, Xiaoyu
Zhang, Qingyou

المصدر

Oxidative Medicine and Cellular Longevity

العدد

المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2020-01-29

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الأحياء

الملخص EN

The regulatory mechanisms for proliferation and migration of vascular smooth muscle cells have not yet been clear.

The present study was designed to investigate whether and how endothelin-1 (ET-1) impacted the generation of endogenous sulfur dioxide (SO2) in rat vascular smooth muscle cell (VSMC) proliferation and migration.

Primary VSMCs and purified aspartate aminotransferase (AAT) protein were used in this study.

We found that in the presence of ET-1, the expression of PCNA and Ki-67 was upregulated and the migration of VSMCs was promoted, while the AAT activity and SO2 levels in VSMCs were reduced without any changes in AAT1 and AAT2 expression.

SO2 supplementation successfully prevented the ET-1-facilitated expression of PCNA and Ki-67 and the migration of VSMCs.

Interestingly, ET-1 significantly increased reactive oxygen species (ROS) production in association with SO2/AAT pathway downregulation in VSMCs compared with controls, while the ROS scavenger N-acetyl-L-cysteine (NAC) and the antioxidant glutathione (GSH) significantly abolished the ET-1-stimulated downregulation of the SO2/AAT pathway.

Moreover, the AAT activity was reduced in purified protein after the treatment for 2 h.

However, NAC and GSH blocked the hydrogen peroxide-induced AAT activity reduction.

In conclusion, our results suggest that ET-1 results in the downregulation of the endogenous SO2/AAT pathway via ROS generation to enhance the proliferation and migration of VSMCs.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Tian, Xiaoyu& Zhang, Qingyou& Huang, Yaqian& Chen, Selena& Tang, Chaoshu& Sun, Yan…[et al.]. 2020. Endothelin-1 Downregulates Sulfur DioxideAspartate Aminotransferase Pathway via Reactive Oxygen Species to Promote the Proliferation and Migration of Vascular Smooth Muscle Cells. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1206034

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Tian, Xiaoyu…[et al.]. Endothelin-1 Downregulates Sulfur DioxideAspartate Aminotransferase Pathway via Reactive Oxygen Species to Promote the Proliferation and Migration of Vascular Smooth Muscle Cells. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1206034

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Tian, Xiaoyu& Zhang, Qingyou& Huang, Yaqian& Chen, Selena& Tang, Chaoshu& Sun, Yan…[et al.]. Endothelin-1 Downregulates Sulfur DioxideAspartate Aminotransferase Pathway via Reactive Oxygen Species to Promote the Proliferation and Migration of Vascular Smooth Muscle Cells. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1206034

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1206034