Endothelin-1 Downregulates Sulfur DioxideAspartate Aminotransferase Pathway via Reactive Oxygen Species to Promote the Proliferation and Migration of Vascular Smooth Muscle Cells
Joint Authors
Junbao, D.
Huang, Yaqian
Sun, Yan
Tang, Chaoshu
Jin, Hongfang
Chen, Selena
Tian, Xiaoyu
Zhang, Qingyou
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The regulatory mechanisms for proliferation and migration of vascular smooth muscle cells have not yet been clear.
The present study was designed to investigate whether and how endothelin-1 (ET-1) impacted the generation of endogenous sulfur dioxide (SO2) in rat vascular smooth muscle cell (VSMC) proliferation and migration.
Primary VSMCs and purified aspartate aminotransferase (AAT) protein were used in this study.
We found that in the presence of ET-1, the expression of PCNA and Ki-67 was upregulated and the migration of VSMCs was promoted, while the AAT activity and SO2 levels in VSMCs were reduced without any changes in AAT1 and AAT2 expression.
SO2 supplementation successfully prevented the ET-1-facilitated expression of PCNA and Ki-67 and the migration of VSMCs.
Interestingly, ET-1 significantly increased reactive oxygen species (ROS) production in association with SO2/AAT pathway downregulation in VSMCs compared with controls, while the ROS scavenger N-acetyl-L-cysteine (NAC) and the antioxidant glutathione (GSH) significantly abolished the ET-1-stimulated downregulation of the SO2/AAT pathway.
Moreover, the AAT activity was reduced in purified protein after the treatment for 2 h.
However, NAC and GSH blocked the hydrogen peroxide-induced AAT activity reduction.
In conclusion, our results suggest that ET-1 results in the downregulation of the endogenous SO2/AAT pathway via ROS generation to enhance the proliferation and migration of VSMCs.
American Psychological Association (APA)
Tian, Xiaoyu& Zhang, Qingyou& Huang, Yaqian& Chen, Selena& Tang, Chaoshu& Sun, Yan…[et al.]. 2020. Endothelin-1 Downregulates Sulfur DioxideAspartate Aminotransferase Pathway via Reactive Oxygen Species to Promote the Proliferation and Migration of Vascular Smooth Muscle Cells. Oxidative Medicine and Cellular Longevity،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1206034
Modern Language Association (MLA)
Tian, Xiaoyu…[et al.]. Endothelin-1 Downregulates Sulfur DioxideAspartate Aminotransferase Pathway via Reactive Oxygen Species to Promote the Proliferation and Migration of Vascular Smooth Muscle Cells. Oxidative Medicine and Cellular Longevity No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1206034
American Medical Association (AMA)
Tian, Xiaoyu& Zhang, Qingyou& Huang, Yaqian& Chen, Selena& Tang, Chaoshu& Sun, Yan…[et al.]. Endothelin-1 Downregulates Sulfur DioxideAspartate Aminotransferase Pathway via Reactive Oxygen Species to Promote the Proliferation and Migration of Vascular Smooth Muscle Cells. Oxidative Medicine and Cellular Longevity. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1206034
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1206034