Vitamin C Treatment Rescues Prelamin A-Induced Premature Senescence of Subchondral Bone Mesenchymal Stem Cells
المؤلفون المشاركون
Jiang, Xiao-Xia
Qu, Yan-Nv
Zhang, Li
Wang, Ting
Zhang, He-Yang
Yang, Ze-Ji
Yuan, Fang-Fang
Wang, Yan
Li, Si-Wei
Xie, Xiao-Hua
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-04-03
دولة النشر
مصر
عدد الصفحات
16
الملخص EN
Aging is a predominant risk factor for many chronic conditions.
Stem cell dysfunction plays a pivotal role in the aging process.
Prelamin A, an abnormal processed form of the nuclear lamina protein lamin A, has been reported to trigger premature senescence.
However, the mechanism driving stem cell dysfunction is still unclear.
In this study, we found that while passaging subchondral bone mesenchymal stem cells (SCB-MSCs) in vitro, prelamin A accumulation occurred concomitantly with an increase in senescence-associated β-galactosidase (SA-β-Gal) expression.
Unlike their counterparts, SCB-MSCs with prelamin A overexpression (MSC/PLA) demonstrated decreased proliferation, osteogenesis, and adipogenesis but increased production of inflammatory factors.
In a hind-limb ischemia model, MSC/PLA also exhibited compromised therapy effect.
Further investigation showed that exogenous prelamin A triggered abnormal nuclear morphology, DNA and shelterin complex damage, cell cycle retardation, and eventually cell senescence.
Changes in gene expression profile were also verified by microarray assay.
Interestingly, we found that ascorbic acid or vitamin C (VC) treatment could inhibit prelamin A expression in MSC/PLA and partially reverse the premature aging in MSC/PLA, with reduced secretion of inflammatory factors and cell cycle arrest and resistance to apoptosis.
Importantly, after VC treatment, MSC/PLA showed enhanced therapy effect in the hind-limb ischemia model.
In conclusion, prelamin A can accelerate SCB-MSC premature senescence by inducing DNA damage.
VC can be a potential therapeutic reagent for prelamin A-induced aging defects in MSCs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Qu, Yan-Nv& Zhang, Li& Wang, Ting& Zhang, He-Yang& Yang, Ze-Ji& Yuan, Fang-Fang…[et al.]. 2020. Vitamin C Treatment Rescues Prelamin A-Induced Premature Senescence of Subchondral Bone Mesenchymal Stem Cells. Stem Cells International،Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1207693
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Qu, Yan-Nv…[et al.]. Vitamin C Treatment Rescues Prelamin A-Induced Premature Senescence of Subchondral Bone Mesenchymal Stem Cells. Stem Cells International No. 2020 (2020), pp.1-16.
https://search.emarefa.net/detail/BIM-1207693
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Qu, Yan-Nv& Zhang, Li& Wang, Ting& Zhang, He-Yang& Yang, Ze-Ji& Yuan, Fang-Fang…[et al.]. Vitamin C Treatment Rescues Prelamin A-Induced Premature Senescence of Subchondral Bone Mesenchymal Stem Cells. Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1207693
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1207693
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر