Screening Genes Promoting Exit from Naive Pluripotency Based on Genome-Scale CRISPR-Cas9 Knockout
المؤلفون المشاركون
Liu, Nan-Bo
Zhu, Ping
Nasser, M. I.
Zhao, Mingyi
Zhou, Wenyi
Zhu, Shuoji
Yang, Bin
Kuang, Junqi
Wu, Chuman
Jiang, Haodong
Zhai, Ziwei
Wu, Yue
Peng, Junwei
Hu, Haiyan
Chen, Ruiping
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-02-03
دولة النشر
مصر
عدد الصفحات
15
الملخص EN
Two of the main problems of stem cell and regenerative medicine are the exit of pluripotency and differentiation to functional cells or tissues.
The answer to these two problems holds great value in the clinical translation of stem cell as well as regenerative medicine research.
Although piling researches have revealed the truth about pluripotency maintenance, the mechanisms underlying pluripotent cell self-renewal, proliferation, and differentiation into specific cell lineages or tissues are yet to be defined.
To this end, we took full advantage of a novel technology, namely, the genome-scale CRISPR-Cas9 knockout (GeCKO).
As an effective way of introducing targeted loss-of-function mutations at specific sites in the genome, GeCKO is able to screen in an unbiased manner for key genes that promote exit from pluripotency in mouse embryonic stem cells (mESCs) for the first time.
In this study, we successfully established a model based on GeCKO to screen the key genes in pluripotency withdrawal.
Our strategies included lentiviral package and infection technology, lenti-Cas9 gene knockout technology, shRNA gene knockdown technology, next-generation sequencing, model-based analysis of genome-scale CRISPR-Cas9 knockout (MAGeCK analysis), GO analysis, and other methods.
Our findings provide a novel approach for large-scale screening of genes involved in pluripotency exit and offer an entry point for cell fate regulation research.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Bin& Kuang, Junqi& Wu, Chuman& Zhou, Wenyi& Zhu, Shuoji& Jiang, Haodong…[et al.]. 2020. Screening Genes Promoting Exit from Naive Pluripotency Based on Genome-Scale CRISPR-Cas9 Knockout. Stem Cells International،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1207888
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Bin…[et al.]. Screening Genes Promoting Exit from Naive Pluripotency Based on Genome-Scale CRISPR-Cas9 Knockout. Stem Cells International No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1207888
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Bin& Kuang, Junqi& Wu, Chuman& Zhou, Wenyi& Zhu, Shuoji& Jiang, Haodong…[et al.]. Screening Genes Promoting Exit from Naive Pluripotency Based on Genome-Scale CRISPR-Cas9 Knockout. Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1207888
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1207888
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر