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Screening Genes Promoting Exit from Naive Pluripotency Based on Genome-Scale CRISPR-Cas9 Knockout
Joint Authors
Liu, Nan-Bo
Zhu, Ping
Nasser, M. I.
Zhao, Mingyi
Zhou, Wenyi
Zhu, Shuoji
Yang, Bin
Kuang, Junqi
Wu, Chuman
Jiang, Haodong
Zhai, Ziwei
Wu, Yue
Peng, Junwei
Hu, Haiyan
Chen, Ruiping
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-03
Country of Publication
Egypt
No. of Pages
15
Abstract EN
Two of the main problems of stem cell and regenerative medicine are the exit of pluripotency and differentiation to functional cells or tissues.
The answer to these two problems holds great value in the clinical translation of stem cell as well as regenerative medicine research.
Although piling researches have revealed the truth about pluripotency maintenance, the mechanisms underlying pluripotent cell self-renewal, proliferation, and differentiation into specific cell lineages or tissues are yet to be defined.
To this end, we took full advantage of a novel technology, namely, the genome-scale CRISPR-Cas9 knockout (GeCKO).
As an effective way of introducing targeted loss-of-function mutations at specific sites in the genome, GeCKO is able to screen in an unbiased manner for key genes that promote exit from pluripotency in mouse embryonic stem cells (mESCs) for the first time.
In this study, we successfully established a model based on GeCKO to screen the key genes in pluripotency withdrawal.
Our strategies included lentiviral package and infection technology, lenti-Cas9 gene knockout technology, shRNA gene knockdown technology, next-generation sequencing, model-based analysis of genome-scale CRISPR-Cas9 knockout (MAGeCK analysis), GO analysis, and other methods.
Our findings provide a novel approach for large-scale screening of genes involved in pluripotency exit and offer an entry point for cell fate regulation research.
American Psychological Association (APA)
Yang, Bin& Kuang, Junqi& Wu, Chuman& Zhou, Wenyi& Zhu, Shuoji& Jiang, Haodong…[et al.]. 2020. Screening Genes Promoting Exit from Naive Pluripotency Based on Genome-Scale CRISPR-Cas9 Knockout. Stem Cells International،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1207888
Modern Language Association (MLA)
Yang, Bin…[et al.]. Screening Genes Promoting Exit from Naive Pluripotency Based on Genome-Scale CRISPR-Cas9 Knockout. Stem Cells International No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1207888
American Medical Association (AMA)
Yang, Bin& Kuang, Junqi& Wu, Chuman& Zhou, Wenyi& Zhu, Shuoji& Jiang, Haodong…[et al.]. Screening Genes Promoting Exit from Naive Pluripotency Based on Genome-Scale CRISPR-Cas9 Knockout. Stem Cells International. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1207888
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1207888