Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis
المؤلفون المشاركون
Tang, Si-Yuan
Yue, Shaojie
Zhao, Feiyan
Liu, Wei
Yang, Lei
Hua, Qingzhong
Zhou, Yan
Cheng, Haipeng
Luo, Ziqiang
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-01-03
دولة النشر
مصر
عدد الصفحات
13
الملخص EN
Granulocyte colony-stimulating factor (G-CSF) can promote the repair of a variety of damaged tissues, but the underlying mechanisms have not yet been fully elucidated.
Bone marrow mesenchymal stem cells (BM-MSCs) play an important role in the repair of damaged tissue.
The aim of this study was to explore whether pretreating BM-MSCs with G-CSF can promote their ability of homing to the lung after in vitro transplantation via upregulating the CXCR4 expression, potentially markedly increasing the antifibrotic effect of BM-MSCs.
The BM-MSCs pretreated with G-CSF were transplanted into a mouse on day 14 after bleomycin injection.
The antifibrotic effects of BM-MSCs in mice were tested on day 21 by using pathological examination and collagen content assay.
Pretreatment of BM-MSCs with G-CSF significantly promoted their ability of homing to the lung and enhanced their antifibrotic effects.
However, knocking down the CXCR4 expression in BM-MSCs significantly inhibited the ability of G-CSF to promote the migration and homing of BM-MSCs to the lung and the resulting antifibrotic effects.
We also found that G-CSF significantly increased the CXCR4 expression and AKT phosphorylation in BM-MSCs, and the AKT pathway inhibitor LY294002 significantly diminished the ability of G-CSF to upregulate the CXCR4 expression in BM-MSCs.
Pretreatment of BM-MSCs with G-CSF promotes the homing of BM-MSCs to the lung via upregulating the CXCR4 expression, leading to a marked increase in the antifibrotic effects of BM-MSCs.
This study provides new avenues for the application of BM-MSCs in the repair of different tissues.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhao, Feiyan& Liu, Wei& Yue, Shaojie& Yang, Lei& Hua, Qingzhong& Zhou, Yan…[et al.]. 2019. Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis. Stem Cells International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1208290
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhao, Feiyan…[et al.]. Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis. Stem Cells International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1208290
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhao, Feiyan& Liu, Wei& Yue, Shaojie& Yang, Lei& Hua, Qingzhong& Zhou, Yan…[et al.]. Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1208290
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1208290
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر