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Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis
Joint Authors
Tang, Si-Yuan
Yue, Shaojie
Zhao, Feiyan
Liu, Wei
Yang, Lei
Hua, Qingzhong
Zhou, Yan
Cheng, Haipeng
Luo, Ziqiang
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-03
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Granulocyte colony-stimulating factor (G-CSF) can promote the repair of a variety of damaged tissues, but the underlying mechanisms have not yet been fully elucidated.
Bone marrow mesenchymal stem cells (BM-MSCs) play an important role in the repair of damaged tissue.
The aim of this study was to explore whether pretreating BM-MSCs with G-CSF can promote their ability of homing to the lung after in vitro transplantation via upregulating the CXCR4 expression, potentially markedly increasing the antifibrotic effect of BM-MSCs.
The BM-MSCs pretreated with G-CSF were transplanted into a mouse on day 14 after bleomycin injection.
The antifibrotic effects of BM-MSCs in mice were tested on day 21 by using pathological examination and collagen content assay.
Pretreatment of BM-MSCs with G-CSF significantly promoted their ability of homing to the lung and enhanced their antifibrotic effects.
However, knocking down the CXCR4 expression in BM-MSCs significantly inhibited the ability of G-CSF to promote the migration and homing of BM-MSCs to the lung and the resulting antifibrotic effects.
We also found that G-CSF significantly increased the CXCR4 expression and AKT phosphorylation in BM-MSCs, and the AKT pathway inhibitor LY294002 significantly diminished the ability of G-CSF to upregulate the CXCR4 expression in BM-MSCs.
Pretreatment of BM-MSCs with G-CSF promotes the homing of BM-MSCs to the lung via upregulating the CXCR4 expression, leading to a marked increase in the antifibrotic effects of BM-MSCs.
This study provides new avenues for the application of BM-MSCs in the repair of different tissues.
American Psychological Association (APA)
Zhao, Feiyan& Liu, Wei& Yue, Shaojie& Yang, Lei& Hua, Qingzhong& Zhou, Yan…[et al.]. 2019. Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis. Stem Cells International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1208290
Modern Language Association (MLA)
Zhao, Feiyan…[et al.]. Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis. Stem Cells International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1208290
American Medical Association (AMA)
Zhao, Feiyan& Liu, Wei& Yue, Shaojie& Yang, Lei& Hua, Qingzhong& Zhou, Yan…[et al.]. Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1208290
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1208290