Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells
المؤلفون المشاركون
Lee, Jun Ho
Oh, Seung Ha
Suh, Myung-Whan
Kim, So Young
Park, Moo Kyun
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-14
دولة النشر
مصر
عدد الصفحات
7
الملخص EN
This study was aimed at investigating the effects of placenta-derived mesenchymal stem cells (PL-MSCs) on particulate matter- (PM-) exposed human middle ear epithelial cells (HMEECs).
HMEECs were treated with 300 μg/ml PM for 24 hours.
The PL-MSCs were cocultured with PM-treated HMEECs.
Cells were harvested on days 0, 1, and 4, and the expression of the inflammatory genes TNFα, COX2, IL1β, IL6, and MUC5B in HMEECs and anti-inflammatory genes PTGES, TGFβ, and VEGF in PL-MSCs was examined by qRT-PCR.
The culture media were collected to measure the secreted PGE2 level using an enzyme-linked immunosorbent assay.
The mRNA expression of TNFα, COX2, IL1β, IL6, and MUC5B in HMEECs increased following PM treatment.
PM-treated HMEECs cocultured with PL-MSCs showed alleviated inflammatory reactions represented by lower mRNA expression levels of MUC5B, TNFα, IL1β, and IL6 compared to monocultured PM-treated HMEECs.
The mRNA expression levels of PGE2, TGFβ, and VEGF were elevated in cocultured PL-MSCs compared to those of control PL-MSCs.
The medium of PM-treated HMEECs cocultured with PL-MSCs exhibited increased PGE2 levels.
The increased inflammatory response in PM-treated HMEECs was reversed using PL-MSCs.
The PGE2, TGFβ, and VEGF were the mediators of the anti-inflammatory effects of PL-MSCs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kim, So Young& Oh, Seung Ha& Lee, Jun Ho& Suh, Myung-Whan& Park, Moo Kyun. 2019. Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1208843
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kim, So Young…[et al.]. Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells. Stem Cells International No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1208843
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kim, So Young& Oh, Seung Ha& Lee, Jun Ho& Suh, Myung-Whan& Park, Moo Kyun. Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1208843
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1208843
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر