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Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells
Joint Authors
Lee, Jun Ho
Oh, Seung Ha
Suh, Myung-Whan
Kim, So Young
Park, Moo Kyun
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-14
Country of Publication
Egypt
No. of Pages
7
Abstract EN
This study was aimed at investigating the effects of placenta-derived mesenchymal stem cells (PL-MSCs) on particulate matter- (PM-) exposed human middle ear epithelial cells (HMEECs).
HMEECs were treated with 300 μg/ml PM for 24 hours.
The PL-MSCs were cocultured with PM-treated HMEECs.
Cells were harvested on days 0, 1, and 4, and the expression of the inflammatory genes TNFα, COX2, IL1β, IL6, and MUC5B in HMEECs and anti-inflammatory genes PTGES, TGFβ, and VEGF in PL-MSCs was examined by qRT-PCR.
The culture media were collected to measure the secreted PGE2 level using an enzyme-linked immunosorbent assay.
The mRNA expression of TNFα, COX2, IL1β, IL6, and MUC5B in HMEECs increased following PM treatment.
PM-treated HMEECs cocultured with PL-MSCs showed alleviated inflammatory reactions represented by lower mRNA expression levels of MUC5B, TNFα, IL1β, and IL6 compared to monocultured PM-treated HMEECs.
The mRNA expression levels of PGE2, TGFβ, and VEGF were elevated in cocultured PL-MSCs compared to those of control PL-MSCs.
The medium of PM-treated HMEECs cocultured with PL-MSCs exhibited increased PGE2 levels.
The increased inflammatory response in PM-treated HMEECs was reversed using PL-MSCs.
The PGE2, TGFβ, and VEGF were the mediators of the anti-inflammatory effects of PL-MSCs.
American Psychological Association (APA)
Kim, So Young& Oh, Seung Ha& Lee, Jun Ho& Suh, Myung-Whan& Park, Moo Kyun. 2019. Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells. Stem Cells International،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1208843
Modern Language Association (MLA)
Kim, So Young…[et al.]. Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells. Stem Cells International No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1208843
American Medical Association (AMA)
Kim, So Young& Oh, Seung Ha& Lee, Jun Ho& Suh, Myung-Whan& Park, Moo Kyun. Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells. Stem Cells International. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1208843
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1208843